Protocol for open-source automated universal high-content multiplex fluorescence for RNA in situ analysis

Protocol for open-source automated universal high-content multiplex fluorescence for RNA in situ analysis

Summary: In situ hybridization visualizes RNA in cells, but image analysis is complex. We present a protocol based on open-source software for automated high-content multiplex fluorescence in situ transcriptomics analysis. Steps include nuclei segmentation with a Fiji macro and quantification of up...

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Bibliographic Details
Main Authors: Jean Descarpentrie, Florian Bernard, Wilfried Souleyreau, Lucie Brisson, Thomas Mathivet, Ioannis S. Pateras, Océane C.B. Martin, Maria Lopez Chiloeches, Teresa Frisan
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
cell biology
health sciences
in situ hybridization
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724006737
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http://www.sciencedirect.com/science/article/pii/S2666166724006737

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