Adaptation of the HEp-2 cell line to totally animal-free culture systems and real-time analysis of cell growth
Routine cell culture demands the use of animal-derived products, mainly fetal bovine serum and swine or bovine trypsin. According to the 3Rs principle and to the European Centre for the Validation of Alternative Methods, animal-free substitutes are strongly recommended for in vitro methods. In this...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-06-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/btn-2020-0162 |
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| author | Mara Fusi Silvia Dotti |
| author_facet | Mara Fusi Silvia Dotti |
| author_sort | Mara Fusi |
| collection | DOAJ |
| description | Routine cell culture demands the use of animal-derived products, mainly fetal bovine serum and swine or bovine trypsin. According to the 3Rs principle and to the European Centre for the Validation of Alternative Methods, animal-free substitutes are strongly recommended for in vitro methods. In this study, the HEp-2 cell line was adapted to different totally animal-free culture systems, such as a serum-free complete medium (VP-SFM), human platelet lysate and a synthetic trypsin (TrypLE™ Express); afterward, cell growth was assessed with the xCELLigence instrument. Animal-free products provided promising results, with performances similar or preferable to the common reagents; therefore their use could be encouraged for both ethical and technical advantages. |
| format | Article |
| id | doaj-art-4b8c409c15a647f2b7affce5b571cba7 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2021-06-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-4b8c409c15a647f2b7affce5b571cba72025-08-20T02:25:56ZengTaylor & Francis GroupBioTechniques0736-62051940-98182021-06-0170631932610.2144/btn-2020-0162Adaptation of the HEp-2 cell line to totally animal-free culture systems and real-time analysis of cell growthMara Fusi0Silvia Dotti11Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, via Bianchi 9, 25124, Brescia, Italy1Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, via Bianchi 9, 25124, Brescia, ItalyRoutine cell culture demands the use of animal-derived products, mainly fetal bovine serum and swine or bovine trypsin. According to the 3Rs principle and to the European Centre for the Validation of Alternative Methods, animal-free substitutes are strongly recommended for in vitro methods. In this study, the HEp-2 cell line was adapted to different totally animal-free culture systems, such as a serum-free complete medium (VP-SFM), human platelet lysate and a synthetic trypsin (TrypLE™ Express); afterward, cell growth was assessed with the xCELLigence instrument. Animal-free products provided promising results, with performances similar or preferable to the common reagents; therefore their use could be encouraged for both ethical and technical advantages.https://www.future-science.com/doi/10.2144/btn-2020-0162alternative methodsanimal-freecell cultureHEp-2platelet lysatereal-time cell analysis |
| spellingShingle | Mara Fusi Silvia Dotti Adaptation of the HEp-2 cell line to totally animal-free culture systems and real-time analysis of cell growth BioTechniques alternative methods animal-free cell culture HEp-2 platelet lysate real-time cell analysis |
| title | Adaptation of the HEp-2 cell line to totally animal-free culture systems and real-time analysis of cell growth |
| title_full | Adaptation of the HEp-2 cell line to totally animal-free culture systems and real-time analysis of cell growth |
| title_fullStr | Adaptation of the HEp-2 cell line to totally animal-free culture systems and real-time analysis of cell growth |
| title_full_unstemmed | Adaptation of the HEp-2 cell line to totally animal-free culture systems and real-time analysis of cell growth |
| title_short | Adaptation of the HEp-2 cell line to totally animal-free culture systems and real-time analysis of cell growth |
| title_sort | adaptation of the hep 2 cell line to totally animal free culture systems and real time analysis of cell growth |
| topic | alternative methods animal-free cell culture HEp-2 platelet lysate real-time cell analysis |
| url | https://www.future-science.com/doi/10.2144/btn-2020-0162 |
| work_keys_str_mv | AT marafusi adaptationofthehep2celllinetototallyanimalfreeculturesystemsandrealtimeanalysisofcellgrowth AT silviadotti adaptationofthehep2celllinetototallyanimalfreeculturesystemsandrealtimeanalysisofcellgrowth |