Adaptation of the HEp-2 cell line to totally animal-free culture systems and real-time analysis of cell growth

Routine cell culture demands the use of animal-derived products, mainly fetal bovine serum and swine or bovine trypsin. According to the 3Rs principle and to the European Centre for the Validation of Alternative Methods, animal-free substitutes are strongly recommended for in vitro methods. In this...

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Bibliographic Details
Main Authors: Mara Fusi, Silvia Dotti
Format: Article
Language:English
Published: Taylor & Francis Group 2021-06-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/btn-2020-0162
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