Sporadic detection of vaccine-derived poliovirus type 2 using next-generation sequencing in Canadian wastewater in August of 2022
Abstract In July 2022, an unvaccinated young adult living in Rockland County, New York became paralyzed due to a vaccine-derived poliovirus type 2 (VDPV2) infection that was internationally transmitted. Wastewater surveillance in New York State uncovered VDPV2 in neighboring counties, showing silent...
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Nature Portfolio
2025-04-01
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| Online Access: | https://doi.org/10.1038/s41598-025-92912-x |
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| author | Grace E. Seo Russell Mandes Nathan D. Wright Justin P. Hawkins Anneliese Landgraff Ravinder Lidder Umar Mohammed Chand S. Mangat Anne-Sophie Michel Judith Fafard Darian Hole Andrea D. Tyler Marli Vlok Elsie Grudeski Tim F. Booth Anna Majer |
| author_facet | Grace E. Seo Russell Mandes Nathan D. Wright Justin P. Hawkins Anneliese Landgraff Ravinder Lidder Umar Mohammed Chand S. Mangat Anne-Sophie Michel Judith Fafard Darian Hole Andrea D. Tyler Marli Vlok Elsie Grudeski Tim F. Booth Anna Majer |
| author_sort | Grace E. Seo |
| collection | DOAJ |
| description | Abstract In July 2022, an unvaccinated young adult living in Rockland County, New York became paralyzed due to a vaccine-derived poliovirus type 2 (VDPV2) infection that was internationally transmitted. Wastewater surveillance in New York State uncovered VDPV2 in neighboring counties, showing silent community spread. These communities have strong epidemiological links to some vaccine-hesitant communities in Canada, spurring the need to monitor these populations for potential poliovirus importation. At the time, Canada did not have an established poliovirus wastewater method. We initiated this study to apply molecular methods to detect poliovirus in these communities and establish poliovirus wastewater surveillance capabilities in Canada. We sequence confirmed one poliovirus detection on August 30th, 2022 using both viral isolation and direct detection methods. Subsequent retrospective and prospective sampling was initiated, resulting in another sequence confirmed detection of VDPV2 in an overlapping catchment area collected on August 27th, 2022. Both VDPV2 detections were genetically linked to the New York clinical case. No clinical cases of poliomyelitis were detected in Canada during this study. The sporadic detection of VDPV2 in Canadian wastewater supports a travel-related shedding event without community transmission. Furthermore, we show that a direct detection method is sensitive to sequence confirm poliovirus in wastewater samples. |
| format | Article |
| id | doaj-art-4b4ccadc8998461d8f507de6d800c2c1 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-4b4ccadc8998461d8f507de6d800c2c12025-08-20T02:27:53ZengNature PortfolioScientific Reports2045-23222025-04-0115111010.1038/s41598-025-92912-xSporadic detection of vaccine-derived poliovirus type 2 using next-generation sequencing in Canadian wastewater in August of 2022Grace E. Seo0Russell Mandes1Nathan D. Wright2Justin P. Hawkins3Anneliese Landgraff4Ravinder Lidder5Umar Mohammed6Chand S. Mangat7Anne-Sophie Michel8Judith Fafard9Darian Hole10Andrea D. Tyler11Marli Vlok12Elsie Grudeski13Tim F. Booth14Anna Majer15Enterovirus and Enteric Viruses Laboratory, Viral Diseases, National Microbiology Laboratory, Public Health Agency of CanadaEnterovirus and Enteric Viruses Laboratory, Viral Diseases, National Microbiology Laboratory, Public Health Agency of CanadaEnterovirus and Enteric Viruses Laboratory, Viral Diseases, National Microbiology Laboratory, Public Health Agency of CanadaEnterovirus and Enteric Viruses Laboratory, Viral Diseases, National Microbiology Laboratory, Public Health Agency of CanadaEnterovirus and Enteric Viruses Laboratory, Viral Diseases, National Microbiology Laboratory, Public Health Agency of CanadaWastewater Surveillance Unit, National Microbiology Laboratory, Public Health Agency of CanadaWastewater Surveillance Unit, National Microbiology Laboratory, Public Health Agency of CanadaWastewater Surveillance Unit, National Microbiology Laboratory, Public Health Agency of CanadaMinistère de la Santé et des services sociaux du QuébecLaboratoire de santé Publique du Québec, Institut national de santé publique du QuébecComputational and Operational Genomics, National Microbiology Laboratory, Public Health Agency of CanadaComputational and Operational Genomics, National Microbiology Laboratory, Public Health Agency of CanadaEnterovirus and Enteric Viruses Laboratory, Viral Diseases, National Microbiology Laboratory, Public Health Agency of CanadaEnterovirus and Enteric Viruses Laboratory, Viral Diseases, National Microbiology Laboratory, Public Health Agency of CanadaEnterovirus and Enteric Viruses Laboratory, Viral Diseases, National Microbiology Laboratory, Public Health Agency of CanadaEnterovirus and Enteric Viruses Laboratory, Viral Diseases, National Microbiology Laboratory, Public Health Agency of CanadaAbstract In July 2022, an unvaccinated young adult living in Rockland County, New York became paralyzed due to a vaccine-derived poliovirus type 2 (VDPV2) infection that was internationally transmitted. Wastewater surveillance in New York State uncovered VDPV2 in neighboring counties, showing silent community spread. These communities have strong epidemiological links to some vaccine-hesitant communities in Canada, spurring the need to monitor these populations for potential poliovirus importation. At the time, Canada did not have an established poliovirus wastewater method. We initiated this study to apply molecular methods to detect poliovirus in these communities and establish poliovirus wastewater surveillance capabilities in Canada. We sequence confirmed one poliovirus detection on August 30th, 2022 using both viral isolation and direct detection methods. Subsequent retrospective and prospective sampling was initiated, resulting in another sequence confirmed detection of VDPV2 in an overlapping catchment area collected on August 27th, 2022. Both VDPV2 detections were genetically linked to the New York clinical case. No clinical cases of poliomyelitis were detected in Canada during this study. The sporadic detection of VDPV2 in Canadian wastewater supports a travel-related shedding event without community transmission. Furthermore, we show that a direct detection method is sensitive to sequence confirm poliovirus in wastewater samples.https://doi.org/10.1038/s41598-025-92912-xPoliovirusVaccine-derived poliovirus type 2 (VDPV2)Wastewater surveillanceNext generation sequencingNanoporeDirect detection |
| spellingShingle | Grace E. Seo Russell Mandes Nathan D. Wright Justin P. Hawkins Anneliese Landgraff Ravinder Lidder Umar Mohammed Chand S. Mangat Anne-Sophie Michel Judith Fafard Darian Hole Andrea D. Tyler Marli Vlok Elsie Grudeski Tim F. Booth Anna Majer Sporadic detection of vaccine-derived poliovirus type 2 using next-generation sequencing in Canadian wastewater in August of 2022 Scientific Reports Poliovirus Vaccine-derived poliovirus type 2 (VDPV2) Wastewater surveillance Next generation sequencing Nanopore Direct detection |
| title | Sporadic detection of vaccine-derived poliovirus type 2 using next-generation sequencing in Canadian wastewater in August of 2022 |
| title_full | Sporadic detection of vaccine-derived poliovirus type 2 using next-generation sequencing in Canadian wastewater in August of 2022 |
| title_fullStr | Sporadic detection of vaccine-derived poliovirus type 2 using next-generation sequencing in Canadian wastewater in August of 2022 |
| title_full_unstemmed | Sporadic detection of vaccine-derived poliovirus type 2 using next-generation sequencing in Canadian wastewater in August of 2022 |
| title_short | Sporadic detection of vaccine-derived poliovirus type 2 using next-generation sequencing in Canadian wastewater in August of 2022 |
| title_sort | sporadic detection of vaccine derived poliovirus type 2 using next generation sequencing in canadian wastewater in august of 2022 |
| topic | Poliovirus Vaccine-derived poliovirus type 2 (VDPV2) Wastewater surveillance Next generation sequencing Nanopore Direct detection |
| url | https://doi.org/10.1038/s41598-025-92912-x |
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