Lyophyllum decastes-derived polysaccharides alleviate DSS-induced colitis in mice by suppressing inflammation, enhancing intestinal barrier integrity, and restoring gut microbiota homeostasis

IntroductionInflammatory bowel disease (IBD), including ulcerative colitis (UC), is characterized by disturbances in the intestinal barrier, immune system dysfunction, and an altered gut microbiota composition. Lyophyllum decastes, a medicinal mushroom known for its bioactive polysaccharides, has sh...

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Main Authors: Eslam Ghaleb, Yamina Alioui, Jamalat Yazeed, Natheer Wahan, Ahmed Bashah, Ali AL-waqeerah, Aamna Atta, Yuxin Sun, Yi Xin, Liang Wang, Bin Feng, Weifeng Mao
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Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Pharmacology
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Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2025.1644325/full
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author Eslam Ghaleb
Yamina Alioui
Jamalat Yazeed
Natheer Wahan
Ahmed Bashah
Ali AL-waqeerah
Aamna Atta
Yuxin Sun
Yi Xin
Liang Wang
Bin Feng
Weifeng Mao
author_facet Eslam Ghaleb
Yamina Alioui
Jamalat Yazeed
Natheer Wahan
Ahmed Bashah
Ali AL-waqeerah
Aamna Atta
Yuxin Sun
Yi Xin
Liang Wang
Bin Feng
Weifeng Mao
author_sort Eslam Ghaleb
collection DOAJ
description IntroductionInflammatory bowel disease (IBD), including ulcerative colitis (UC), is characterized by disturbances in the intestinal barrier, immune system dysfunction, and an altered gut microbiota composition. Lyophyllum decastes, a medicinal mushroom known for its bioactive polysaccharides, has shown potential anti-inflammatory properties. However, its therapeutic effects on ulcerative colitis have not been studied. This study investigates the effects of L. decastes polysaccharides (LDP) on dextran sulfate sodium (DSS)-induced colitis in mice.MethodsUlcerative colitis was induced in BALB/c mice by administering 3% DSS in drinking water for 7 days. Mice were then treated orally with LDP at doses of 200 or 400 mg/kg for 14 days. Disease activity index (DAI), histopathological analysis, cytokine levels, myeloperoxidase (MPO) activity, tight junction protein expression (occludin and ZO-1), and gut microbiota composition were assessed.Results and discussionLDP treatment significantly reduced DAI scores and preserved colonic histological structure. It modulated cytokine levels, decreasing pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) and increasing anti-inflammatory cytokines (IL-10, IL-4, TGF-β). Additionally, LDP improved intestinal barrier function by reducing MPO activity and enhancing occludin and ZO-1 expression. 16S rRNA sequencing revealed a significant restoration of gut microbiota diversity, with an increase in beneficial bacteria Muribaculaceae, Lactobacillus, and Lachnospiraceae, and a reduction in pathogenic bacteria Escherichia-Shigella. these findings suggest that LDP exhibits therapeutic effects in DSS-induced colitis through anti-inflammatory properties, enhancement of intestinal barrier function, and modulation of gut microbiota. These findings suggest that LDP may serve as a promising novel therapeutic agent for the management of ulcerative colitis.
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spelling doaj-art-4abf28973387478ea9e0ab60753e6fc42025-08-20T02:35:40ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-07-011610.3389/fphar.2025.16443251644325Lyophyllum decastes-derived polysaccharides alleviate DSS-induced colitis in mice by suppressing inflammation, enhancing intestinal barrier integrity, and restoring gut microbiota homeostasisEslam Ghaleb0Yamina Alioui1Jamalat Yazeed2Natheer Wahan3Ahmed Bashah4Ali AL-waqeerah5Aamna Atta6Yuxin Sun7Yi Xin8Liang Wang9Bin Feng10Weifeng Mao11Biochemistry and Molecular Biology Department, College of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaBitechnology Department, College of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaDepartment of Pharmacology, College of Pharmacy, Dalian Medical University, Dalian, ChinaDepartment of Pharmacology, College of Pharmacy, Dalian Medical University, Dalian, ChinaDepartment of Internal Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, ChinaDepartment of Internal Medicine, The First Affiliated Hospital of Dalian Medical University, Dalian, ChinaBiochemistry and Molecular Biology Department, College of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaBitechnology Department, College of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaBitechnology Department, College of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaNational Joint Engineering Laboratory, Stem Cell Clinical Research Center, Regenerative Medicine Center, The First Affiliated Hospital of Dalian Medical University, Dalian, ChinaBiochemistry and Molecular Biology Department, College of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaBitechnology Department, College of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaIntroductionInflammatory bowel disease (IBD), including ulcerative colitis (UC), is characterized by disturbances in the intestinal barrier, immune system dysfunction, and an altered gut microbiota composition. Lyophyllum decastes, a medicinal mushroom known for its bioactive polysaccharides, has shown potential anti-inflammatory properties. However, its therapeutic effects on ulcerative colitis have not been studied. This study investigates the effects of L. decastes polysaccharides (LDP) on dextran sulfate sodium (DSS)-induced colitis in mice.MethodsUlcerative colitis was induced in BALB/c mice by administering 3% DSS in drinking water for 7 days. Mice were then treated orally with LDP at doses of 200 or 400 mg/kg for 14 days. Disease activity index (DAI), histopathological analysis, cytokine levels, myeloperoxidase (MPO) activity, tight junction protein expression (occludin and ZO-1), and gut microbiota composition were assessed.Results and discussionLDP treatment significantly reduced DAI scores and preserved colonic histological structure. It modulated cytokine levels, decreasing pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) and increasing anti-inflammatory cytokines (IL-10, IL-4, TGF-β). Additionally, LDP improved intestinal barrier function by reducing MPO activity and enhancing occludin and ZO-1 expression. 16S rRNA sequencing revealed a significant restoration of gut microbiota diversity, with an increase in beneficial bacteria Muribaculaceae, Lactobacillus, and Lachnospiraceae, and a reduction in pathogenic bacteria Escherichia-Shigella. these findings suggest that LDP exhibits therapeutic effects in DSS-induced colitis through anti-inflammatory properties, enhancement of intestinal barrier function, and modulation of gut microbiota. These findings suggest that LDP may serve as a promising novel therapeutic agent for the management of ulcerative colitis.https://www.frontiersin.org/articles/10.3389/fphar.2025.1644325/fullLyophyllum decastespolysaccharidesulcerative colitisdextran sulfate sodium (DSS)intestinal barrier integritygut microbiota
spellingShingle Eslam Ghaleb
Yamina Alioui
Jamalat Yazeed
Natheer Wahan
Ahmed Bashah
Ali AL-waqeerah
Aamna Atta
Yuxin Sun
Yi Xin
Liang Wang
Bin Feng
Weifeng Mao
Lyophyllum decastes-derived polysaccharides alleviate DSS-induced colitis in mice by suppressing inflammation, enhancing intestinal barrier integrity, and restoring gut microbiota homeostasis
Frontiers in Pharmacology
Lyophyllum decastes
polysaccharides
ulcerative colitis
dextran sulfate sodium (DSS)
intestinal barrier integrity
gut microbiota
title Lyophyllum decastes-derived polysaccharides alleviate DSS-induced colitis in mice by suppressing inflammation, enhancing intestinal barrier integrity, and restoring gut microbiota homeostasis
title_full Lyophyllum decastes-derived polysaccharides alleviate DSS-induced colitis in mice by suppressing inflammation, enhancing intestinal barrier integrity, and restoring gut microbiota homeostasis
title_fullStr Lyophyllum decastes-derived polysaccharides alleviate DSS-induced colitis in mice by suppressing inflammation, enhancing intestinal barrier integrity, and restoring gut microbiota homeostasis
title_full_unstemmed Lyophyllum decastes-derived polysaccharides alleviate DSS-induced colitis in mice by suppressing inflammation, enhancing intestinal barrier integrity, and restoring gut microbiota homeostasis
title_short Lyophyllum decastes-derived polysaccharides alleviate DSS-induced colitis in mice by suppressing inflammation, enhancing intestinal barrier integrity, and restoring gut microbiota homeostasis
title_sort lyophyllum decastes derived polysaccharides alleviate dss induced colitis in mice by suppressing inflammation enhancing intestinal barrier integrity and restoring gut microbiota homeostasis
topic Lyophyllum decastes
polysaccharides
ulcerative colitis
dextran sulfate sodium (DSS)
intestinal barrier integrity
gut microbiota
url https://www.frontiersin.org/articles/10.3389/fphar.2025.1644325/full
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