QUANTIFICATION OF OCHRATOXIN A IN MOLDAVIAN WINES

The level of the carcinogenic mycotoxin ochratoxin A (OTA) in wines produced in Moldova, including bottled and raw material wines, was analyzed by enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) analysis with fluorescence detection after immunoaffinity col...

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Bibliographic Details
Main Authors: RODICA STURZA, Olga Lazacovici*
Format: Article
Language:English
Published: Alma Mater Publishing House "Vasile Alecsandri" University of Bacau 2017-10-01
Series:Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry
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Online Access:http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201703&vol=3&aid=4622
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Summary:The level of the carcinogenic mycotoxin ochratoxin A (OTA) in wines produced in Moldova, including bottled and raw material wines, was analyzed by enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) analysis with fluorescence detection after immunoaffinity column clean-up. The study was conducted on wine samples from vintage 2006 - 2016. Our results confirm previously published reports that the levels of OTA are considerably higher in red wines than those in white ones. It was found that OTA levels of analyzed samples differ significantly depending on the harvest year of the wine. Thus, 48 % of studied wine samples from vintage 2006 were contaminated with OTA, including 10 % samples with concentration of OTA higher than 2 µg‧L-1. In contrast, levels of OTA detected in samples from 2016, haven’t exceed 0.05 µg‧L-1 (quantification limit of HPLC method).
ISSN:1582-540X
1582-540X