Effect of host microenvironment and bacterial lifestyles on antimicrobial sensitivity and implications for susceptibility testing

Abstract Bacterial infections remain a major global health issue, with antimicrobial resistance (AMR) worsening the crisis. However, treatment failure can occur even when bacteria show antibiotic susceptibility in diagnostic tests. We explore factors such as phenotypic resilience, bacterial lifestyl...

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Main Authors: Ramon Garcia-Maset, Victoria Chu, Nicholas Yuen, Dalia Blumgart, Jenny Yoon, Benjamin O. Murray, Amelia A. Joseph, Jennifer L. Rohn
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:npj Antimicrobials and Resistance
Online Access:https://doi.org/10.1038/s44259-025-00113-3
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author Ramon Garcia-Maset
Victoria Chu
Nicholas Yuen
Dalia Blumgart
Jenny Yoon
Benjamin O. Murray
Amelia A. Joseph
Jennifer L. Rohn
author_facet Ramon Garcia-Maset
Victoria Chu
Nicholas Yuen
Dalia Blumgart
Jenny Yoon
Benjamin O. Murray
Amelia A. Joseph
Jennifer L. Rohn
author_sort Ramon Garcia-Maset
collection DOAJ
description Abstract Bacterial infections remain a major global health issue, with antimicrobial resistance (AMR) worsening the crisis. However, treatment failure can occur even when bacteria show antibiotic susceptibility in diagnostic tests. We explore factors such as phenotypic resilience, bacterial lifestyles such as biofilms, and differences between laboratory tests and real infection sites, highlighting the need for improved platforms to better predict treatment outcomes, and reviewing emerging technologies aimed at improving susceptibility testing.
format Article
id doaj-art-49d9f90c3f0b44da85fbafd0eb45759f
institution OA Journals
issn 2731-8745
language English
publishDate 2025-05-01
publisher Nature Portfolio
record_format Article
series npj Antimicrobials and Resistance
spelling doaj-art-49d9f90c3f0b44da85fbafd0eb45759f2025-08-20T02:34:19ZengNature Portfolionpj Antimicrobials and Resistance2731-87452025-05-013111710.1038/s44259-025-00113-3Effect of host microenvironment and bacterial lifestyles on antimicrobial sensitivity and implications for susceptibility testingRamon Garcia-Maset0Victoria Chu1Nicholas Yuen2Dalia Blumgart3Jenny Yoon4Benjamin O. Murray5Amelia A. Joseph6Jennifer L. Rohn7Centre for Urological Biology, Department of Renal Medicine, Division of Medicine, University College LondonCentre for Urological Biology, Department of Renal Medicine, Division of Medicine, University College LondonCentre for Urological Biology, Department of Renal Medicine, Division of Medicine, University College LondonCentre for Urological Biology, Department of Renal Medicine, Division of Medicine, University College LondonCentre for Urological Biology, Department of Renal Medicine, Division of Medicine, University College LondonCentre for Urological Biology, Department of Renal Medicine, Division of Medicine, University College LondonNottingham University Hospitals NHS TrustCentre for Urological Biology, Department of Renal Medicine, Division of Medicine, University College LondonAbstract Bacterial infections remain a major global health issue, with antimicrobial resistance (AMR) worsening the crisis. However, treatment failure can occur even when bacteria show antibiotic susceptibility in diagnostic tests. We explore factors such as phenotypic resilience, bacterial lifestyles such as biofilms, and differences between laboratory tests and real infection sites, highlighting the need for improved platforms to better predict treatment outcomes, and reviewing emerging technologies aimed at improving susceptibility testing.https://doi.org/10.1038/s44259-025-00113-3
spellingShingle Ramon Garcia-Maset
Victoria Chu
Nicholas Yuen
Dalia Blumgart
Jenny Yoon
Benjamin O. Murray
Amelia A. Joseph
Jennifer L. Rohn
Effect of host microenvironment and bacterial lifestyles on antimicrobial sensitivity and implications for susceptibility testing
npj Antimicrobials and Resistance
title Effect of host microenvironment and bacterial lifestyles on antimicrobial sensitivity and implications for susceptibility testing
title_full Effect of host microenvironment and bacterial lifestyles on antimicrobial sensitivity and implications for susceptibility testing
title_fullStr Effect of host microenvironment and bacterial lifestyles on antimicrobial sensitivity and implications for susceptibility testing
title_full_unstemmed Effect of host microenvironment and bacterial lifestyles on antimicrobial sensitivity and implications for susceptibility testing
title_short Effect of host microenvironment and bacterial lifestyles on antimicrobial sensitivity and implications for susceptibility testing
title_sort effect of host microenvironment and bacterial lifestyles on antimicrobial sensitivity and implications for susceptibility testing
url https://doi.org/10.1038/s44259-025-00113-3
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