Optimizing expression of LZ-8 protein and primary assaying of its immunomodulatory activity
To obtain sufficient recombinant LZ-8 protein (rLZ-8) for assaying its immunosuppressive activity, 12 overlapping oligonucleotides optimized by the usage of the favorite codons in E. coli were synthesized and connected to form a new LZ-8 gene by PCR. The recombinant plasmid pET-28b_lz8 containing th...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2008-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.01.002 |
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