Exploring temperature-dependent transcriptomic adaptations in Yersinia pestis using direct cDNA sequencing by Oxford Nanopore Technologies

Exploring temperature-dependent transcriptomic adaptations in Yersinia pestis using direct cDNA sequencing by Oxford Nanopore Technologies

Abstract Transcriptomics is key to understanding how bacterial pathogens adapt and cause disease, but remains constrained by cost, technical, and biosafety issues, especially for highly virulent and/or regulated pathogens. Here, we present a streamlined and cost-effective RNA-Seq workflow using Oxfo...

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Bibliographic Details
Main Authors: Brandon Robin, Alexandre Baillez, Servane Le Guillouzer, Cécile Lecoeur, Florent Sebbane, Sébastien Bontemps-Gallo
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
Subjects:
Oxford Nanopore Technology
RNA-Seq
Yersinia pestis
Operons mapping
Temperature adaptation
Online Access:https://doi.org/10.1038/s41598-025-05662-1
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https://doi.org/10.1038/s41598-025-05662-1

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