Individual and parentage testing in horses by pcr methodology
<b>Aim:</b> The objective of this study was to develop and test a microsatellite panel for parentage analysis in horses.<p> <b>Materials and Methods:</b> A total of 189 blood samples were collected from four different horse breeds in Turkey. We selected five horse micro...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=851 |
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| author | Mehmet Nizamlıoğlu Ercan Kurar Ercan Zafer Bulut Şeref İnal Ferudun Erzurum |
| author_facet | Mehmet Nizamlıoğlu Ercan Kurar Ercan Zafer Bulut Şeref İnal Ferudun Erzurum |
| author_sort | Mehmet Nizamlıoğlu |
| collection | DOAJ |
| description | <b>Aim:</b> The objective of this study was to develop and test a
microsatellite panel for parentage analysis in horses.<p>
<b>Materials and Methods:</b> A total of 189 blood samples were
collected from four different horse breeds in Turkey. We selected
five horse microsatellite loci and used to amplify genomic
DNA by polymerase chain reaction (PCR). The resulting
PCR products were separated on polyacrylamide gels.
Allele identification was conducted based on their base-pair
size by comparing a size standard.<p>
<b>Results:</b> A total of 53 alleles was determined ranging from
9 to 13 at each locus. The observed heterozygosity (Ho) and
expected heterozygosity (He) were ranged from 0.496 to
0.880 and from 0.800 to 0.851, respectively. Polymorphism
information content (PIC) values were observed between
0.774 and 0.832. Power of exclusion (PE) at each microsatellite
locus ranged from 0.619 to 0.702, resulting in a total
PE value of 0.99060.<p>
<b>Conclusion:</b> These results indicate that this set of microsatellite
is useful for horse parentage testing in Turkey. Due to
possible high level of inbreeding in some breeds, the use of
increased number microsatellite loci will thereby be appropriate
for avoiding a false parenting and misidentification. |
| format | Article |
| id | doaj-art-4855c371c8fb494785ebbf98ce19c7fc |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-4855c371c8fb494785ebbf98ce19c7fc2025-01-02T22:36:24ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532827781851Individual and parentage testing in horses by pcr methodologyMehmet Nizamlıoğlu0Ercan Kurar1Ercan2Zafer Bulut3Şeref İnal4Ferudun Erzurum5Selçuk Üniversitesi, Biyokimya Anabilim Dalı, KonyaSelçuk Üniversitesi, Genetik Anabilim Dalı, Konyaİleri Teknoloji Araştırma ve Uygulama Merkezi (İLTEK)Selçuk Üniversitesi, Biyokimya Anabilim Dalı, KonyaSelçuk Üniversitesi, Zootekni Anabilim Dalı, Veteriner Fakültesi, KonyaGıda, Tarım ve Hayvancılık Bakanlığı, Tarımsal İşletmeler Genel Müdürlüğü, Ankara, Türkiye<b>Aim:</b> The objective of this study was to develop and test a microsatellite panel for parentage analysis in horses.<p> <b>Materials and Methods:</b> A total of 189 blood samples were collected from four different horse breeds in Turkey. We selected five horse microsatellite loci and used to amplify genomic DNA by polymerase chain reaction (PCR). The resulting PCR products were separated on polyacrylamide gels. Allele identification was conducted based on their base-pair size by comparing a size standard.<p> <b>Results:</b> A total of 53 alleles was determined ranging from 9 to 13 at each locus. The observed heterozygosity (Ho) and expected heterozygosity (He) were ranged from 0.496 to 0.880 and from 0.800 to 0.851, respectively. Polymorphism information content (PIC) values were observed between 0.774 and 0.832. Power of exclusion (PE) at each microsatellite locus ranged from 0.619 to 0.702, resulting in a total PE value of 0.99060.<p> <b>Conclusion:</b> These results indicate that this set of microsatellite is useful for horse parentage testing in Turkey. Due to possible high level of inbreeding in some breeds, the use of increased number microsatellite loci will thereby be appropriate for avoiding a false parenting and misidentification.http://eurasianjvetsci.org/pdf.php3?id=851horsemicrosatelliteparentage |
| spellingShingle | Mehmet Nizamlıoğlu Ercan Kurar Ercan Zafer Bulut Şeref İnal Ferudun Erzurum Individual and parentage testing in horses by pcr methodology Eurasian Journal of Veterinary Sciences horse microsatellite parentage |
| title | Individual and parentage testing in horses by pcr methodology |
| title_full | Individual and parentage testing in horses by pcr methodology |
| title_fullStr | Individual and parentage testing in horses by pcr methodology |
| title_full_unstemmed | Individual and parentage testing in horses by pcr methodology |
| title_short | Individual and parentage testing in horses by pcr methodology |
| title_sort | individual and parentage testing in horses by pcr methodology |
| topic | horse microsatellite parentage |
| url | http://eurasianjvetsci.org/pdf.php3?id=851 |
| work_keys_str_mv | AT mehmetnizamlıoglu individualandparentagetestinginhorsesbypcrmethodology AT ercankurar individualandparentagetestinginhorsesbypcrmethodology AT ercan individualandparentagetestinginhorsesbypcrmethodology AT zaferbulut individualandparentagetestinginhorsesbypcrmethodology AT serefinal individualandparentagetestinginhorsesbypcrmethodology AT ferudunerzurum individualandparentagetestinginhorsesbypcrmethodology |