Metabarcoding with Illumina and Oxford Nanopore Technologies provides complementary insights into tree seed mycobiota

Metabarcoding with Illumina and Oxford Nanopore Technologies provides complementary insights into tree seed mycobiota

Abstract Background Culturing of fungi is labor-intensive and reveals limited diversity, while high-throughput sequencing of barcodes (i.e., metabarcoding) enables a simultaneous detection of fungi from multiple environmental samples. Metabarcoding using short-read sequencers, such as Illumina platf...

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Bibliographic Details
Main Authors: Jana Mittelstrass, Renate Heinzelmann, René Eschen, Martin Hartmann, Quirin Kupper, Salome Schneider, Simone Prospero, Iva Franić
Format: Article
Language:English
Published: BMC 2025-05-01
Series:Environmental Microbiome
Subjects:
High-throughput sequencing
Amplicon sequencing
Read length
MinION
ITS
Endophytes
Online Access:https://doi.org/10.1186/s40793-025-00712-7
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https://doi.org/10.1186/s40793-025-00712-7

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