Diagnosis of mycoplasmosis in chicks by pathological and real time-pcr methods
<b>Aim:</b> The purpose of this study was to investigate the suitability of the Real Time-PCR in the diagnosis of mycoplasmosis and to determine the pathologic findings in lungs, air sacs, trachea, hearth, liver and kidney tissues.<p> <b>Materials and Methods:</b> Conju...
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Selcuk University Press
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=852 |
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| author | Mehmet Tuzcu Murat Özmen S. Reyhan Karakoç Nevin Tuzcu Atila Yoldaş |
| author_facet | Mehmet Tuzcu Murat Özmen S. Reyhan Karakoç Nevin Tuzcu Atila Yoldaş |
| author_sort | Mehmet Tuzcu |
| collection | DOAJ |
| description | <b>Aim:</b> The purpose of this study was to investigate the suitability
of the Real Time-PCR in the diagnosis of mycoplasmosis
and to determine the pathologic findings in lungs, air
sacs, trachea, hearth, liver and kidney tissues.<p>
<b>Materials and Methods:</b> Conjunctiva and tracheal swab
samples were taken from broiler chicks with respiratory
disease complaints from 3 different breeders were used.
Ten chicks from three separate flock in each breeders were
collected. Trachea, air sac, lung, liver, kidney, and heart samples
were also collected from the same chicks after necropsy
in order to perform pathological, microbiological and
Real Time-PCR analyses.<p>
<b>Results:</b> Clinically, nasal and conjunctival discharge and
wheezing were observed. Macroscopic examination illustrated
gross catarrhal exudation in trachea and bronchus.
In microscopically, hyperplasia in trachea and bronchus
epithelia, mucus producing cells and mononuclear cellular
infiltration in lamina propria were observed. Mycoplasma
spp. were successfully isolated in the tracheal tissue of 8
broiler chicks. M. gallisepticum specific nucleic acid was
amplified from tissue and swab samples of 22 broiler chicks
by RT-PCR.<p>
<b>Conclusion:</b> RT-PCR seems to be an alternative method
when microbiological analyses are laborious or fails in diagnosis
of mycoplasmosis. |
| format | Article |
| id | doaj-art-47e84c6839d048b185e46e9bef8022c9 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-47e84c6839d048b185e46e9bef8022c92025-02-03T11:25:41ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532828286852Diagnosis of mycoplasmosis in chicks by pathological and real time-pcr methodsMehmet Tuzcu0Murat Özmen1S. Reyhan Karakoç2Nevin Tuzcu3Atila Yoldaş4Department of Pathology, Faculty of Veterinary Medicine, Cumhuriyet University, SivasVeterinary Control and Research Institute, Adana, TurkeyVeterinary Control and Research Institute, Adana, TurkeyDepartment of Research Center, Faculty of Medicine, Cumhuriyet University, SivasVeterinary Control and Research Institute, Adana, Turkey<b>Aim:</b> The purpose of this study was to investigate the suitability of the Real Time-PCR in the diagnosis of mycoplasmosis and to determine the pathologic findings in lungs, air sacs, trachea, hearth, liver and kidney tissues.<p> <b>Materials and Methods:</b> Conjunctiva and tracheal swab samples were taken from broiler chicks with respiratory disease complaints from 3 different breeders were used. Ten chicks from three separate flock in each breeders were collected. Trachea, air sac, lung, liver, kidney, and heart samples were also collected from the same chicks after necropsy in order to perform pathological, microbiological and Real Time-PCR analyses.<p> <b>Results:</b> Clinically, nasal and conjunctival discharge and wheezing were observed. Macroscopic examination illustrated gross catarrhal exudation in trachea and bronchus. In microscopically, hyperplasia in trachea and bronchus epithelia, mucus producing cells and mononuclear cellular infiltration in lamina propria were observed. Mycoplasma spp. were successfully isolated in the tracheal tissue of 8 broiler chicks. M. gallisepticum specific nucleic acid was amplified from tissue and swab samples of 22 broiler chicks by RT-PCR.<p> <b>Conclusion:</b> RT-PCR seems to be an alternative method when microbiological analyses are laborious or fails in diagnosis of mycoplasmosis.http://eurasianjvetsci.org/pdf.php3?id=852mycoplasmosispathologyreal time-pcr |
| spellingShingle | Mehmet Tuzcu Murat Özmen S. Reyhan Karakoç Nevin Tuzcu Atila Yoldaş Diagnosis of mycoplasmosis in chicks by pathological and real time-pcr methods Eurasian Journal of Veterinary Sciences mycoplasmosis pathology real time-pcr |
| title | Diagnosis of mycoplasmosis in chicks by pathological and real time-pcr methods |
| title_full | Diagnosis of mycoplasmosis in chicks by pathological and real time-pcr methods |
| title_fullStr | Diagnosis of mycoplasmosis in chicks by pathological and real time-pcr methods |
| title_full_unstemmed | Diagnosis of mycoplasmosis in chicks by pathological and real time-pcr methods |
| title_short | Diagnosis of mycoplasmosis in chicks by pathological and real time-pcr methods |
| title_sort | diagnosis of mycoplasmosis in chicks by pathological and real time pcr methods |
| topic | mycoplasmosis pathology real time-pcr |
| url | http://eurasianjvetsci.org/pdf.php3?id=852 |
| work_keys_str_mv | AT mehmettuzcu diagnosisofmycoplasmosisinchicksbypathologicalandrealtimepcrmethods AT muratozmen diagnosisofmycoplasmosisinchicksbypathologicalandrealtimepcrmethods AT sreyhankarakoc diagnosisofmycoplasmosisinchicksbypathologicalandrealtimepcrmethods AT nevintuzcu diagnosisofmycoplasmosisinchicksbypathologicalandrealtimepcrmethods AT atilayoldas diagnosisofmycoplasmosisinchicksbypathologicalandrealtimepcrmethods |