Isolation, Identification, and Antimicrobial Susceptibilities of Bacteria from the Conjunctival Sacs of Dogs with Bacterial Conjunctivitis in Different Regions of Wuhan, China
In order to investigate the bacterial species present in the conjunctival sacs of dogs with bacterial conjunctivitis in Wuhan (Hongshan District, Wuchang District, Jiangxia District, and Huangpi District) and their resistance to aminoglycoside antibiotics, samples of conjunctival sac secretions were...
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2025-01-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/12/1/21 |
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| author | Yuxin Li Yinan Wang Xin Gao Lihong Luo Bohan Zhang Xiao Wang Jing Li Ruijia Wu Lixin He Wenxuan Li Changwei Qiu |
| author_facet | Yuxin Li Yinan Wang Xin Gao Lihong Luo Bohan Zhang Xiao Wang Jing Li Ruijia Wu Lixin He Wenxuan Li Changwei Qiu |
| author_sort | Yuxin Li |
| collection | DOAJ |
| description | In order to investigate the bacterial species present in the conjunctival sacs of dogs with bacterial conjunctivitis in Wuhan (Hongshan District, Wuchang District, Jiangxia District, and Huangpi District) and their resistance to aminoglycoside antibiotics, samples of conjunctival sac secretions were collected from 56 dogs with bacterial conjunctivitis in various regions of Wuhan. Drug susceptibility testing for aminoglycoside antibiotics was performed on the most commonly isolated gram-positive and gram-negative bacteria. The expression of two aminoglycoside modifying enzyme genes, <i>aacA-aphD</i> and <i>aac (6′)-Ib</i>, and three 16S rRNA methyltransferase genes, <i>rmtB</i>, <i>rmtE</i> and <i>npmA</i>, were analyzed by PCR. The results showed that a total of 123 bacterial strains were cultured from 56 conjunctival sac secretion samples, with <i>Staphylococcus</i> being the most commonly isolated species, followed by <i>Escherichia</i>. Among them, 14 strains of <i>Staphylococcus pseudointermedius</i> were not resistant to tobramycin, amikacin, gentamicin or neomycin, but the resistance rates to streptomycin and kanamycin were 35.71% and 42.86%, respectively. Among them, 14 <i>Escherichia coli</i> strains were not resistant to tobramycin and gentamicin, but they showed high resistance rates to neomycin and kanamycin (both at 50%). The detection rate of the <i>aacA-aphD</i> gene in <i>Staphylococcus pseudointermedius</i> strains was 100%. The detection rates of the <i>rmtB</i> gene and <i>rmtE</i> gene in <i>Escherichia coli</i> were 85.71% and 28.57%, respectively, while the <i>aac(6′)-Ib</i> gene and <i>npmA</i> gene were not detected. |
| format | Article |
| id | doaj-art-47cb6871403a4f83ae43c0e2926b0c08 |
| institution | Kabale University |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj-art-47cb6871403a4f83ae43c0e2926b0c082025-01-24T13:52:01ZengMDPI AGVeterinary Sciences2306-73812025-01-011212110.3390/vetsci12010021Isolation, Identification, and Antimicrobial Susceptibilities of Bacteria from the Conjunctival Sacs of Dogs with Bacterial Conjunctivitis in Different Regions of Wuhan, ChinaYuxin Li0Yinan Wang1Xin Gao2Lihong Luo3Bohan Zhang4Xiao Wang5Jing Li6Ruijia Wu7Lixin He8Wenxuan Li9Changwei Qiu10Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaIn order to investigate the bacterial species present in the conjunctival sacs of dogs with bacterial conjunctivitis in Wuhan (Hongshan District, Wuchang District, Jiangxia District, and Huangpi District) and their resistance to aminoglycoside antibiotics, samples of conjunctival sac secretions were collected from 56 dogs with bacterial conjunctivitis in various regions of Wuhan. Drug susceptibility testing for aminoglycoside antibiotics was performed on the most commonly isolated gram-positive and gram-negative bacteria. The expression of two aminoglycoside modifying enzyme genes, <i>aacA-aphD</i> and <i>aac (6′)-Ib</i>, and three 16S rRNA methyltransferase genes, <i>rmtB</i>, <i>rmtE</i> and <i>npmA</i>, were analyzed by PCR. The results showed that a total of 123 bacterial strains were cultured from 56 conjunctival sac secretion samples, with <i>Staphylococcus</i> being the most commonly isolated species, followed by <i>Escherichia</i>. Among them, 14 strains of <i>Staphylococcus pseudointermedius</i> were not resistant to tobramycin, amikacin, gentamicin or neomycin, but the resistance rates to streptomycin and kanamycin were 35.71% and 42.86%, respectively. Among them, 14 <i>Escherichia coli</i> strains were not resistant to tobramycin and gentamicin, but they showed high resistance rates to neomycin and kanamycin (both at 50%). The detection rate of the <i>aacA-aphD</i> gene in <i>Staphylococcus pseudointermedius</i> strains was 100%. The detection rates of the <i>rmtB</i> gene and <i>rmtE</i> gene in <i>Escherichia coli</i> were 85.71% and 28.57%, respectively, while the <i>aac(6′)-Ib</i> gene and <i>npmA</i> gene were not detected.https://www.mdpi.com/2306-7381/12/1/21dogsbacterial conjunctivitisaminoglycoside antibioticsantimicrobial susceptibility testresistance genes |
| spellingShingle | Yuxin Li Yinan Wang Xin Gao Lihong Luo Bohan Zhang Xiao Wang Jing Li Ruijia Wu Lixin He Wenxuan Li Changwei Qiu Isolation, Identification, and Antimicrobial Susceptibilities of Bacteria from the Conjunctival Sacs of Dogs with Bacterial Conjunctivitis in Different Regions of Wuhan, China Veterinary Sciences dogs bacterial conjunctivitis aminoglycoside antibiotics antimicrobial susceptibility test resistance genes |
| title | Isolation, Identification, and Antimicrobial Susceptibilities of Bacteria from the Conjunctival Sacs of Dogs with Bacterial Conjunctivitis in Different Regions of Wuhan, China |
| title_full | Isolation, Identification, and Antimicrobial Susceptibilities of Bacteria from the Conjunctival Sacs of Dogs with Bacterial Conjunctivitis in Different Regions of Wuhan, China |
| title_fullStr | Isolation, Identification, and Antimicrobial Susceptibilities of Bacteria from the Conjunctival Sacs of Dogs with Bacterial Conjunctivitis in Different Regions of Wuhan, China |
| title_full_unstemmed | Isolation, Identification, and Antimicrobial Susceptibilities of Bacteria from the Conjunctival Sacs of Dogs with Bacterial Conjunctivitis in Different Regions of Wuhan, China |
| title_short | Isolation, Identification, and Antimicrobial Susceptibilities of Bacteria from the Conjunctival Sacs of Dogs with Bacterial Conjunctivitis in Different Regions of Wuhan, China |
| title_sort | isolation identification and antimicrobial susceptibilities of bacteria from the conjunctival sacs of dogs with bacterial conjunctivitis in different regions of wuhan china |
| topic | dogs bacterial conjunctivitis aminoglycoside antibiotics antimicrobial susceptibility test resistance genes |
| url | https://www.mdpi.com/2306-7381/12/1/21 |
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