Assessing and avoiding C isotopic contamination artefacts in mesocosm-scale 13CO2/12CO2 labelling systems: from biomass components to purified carbohydrates and dark respiration

Assessing and avoiding C isotopic contamination artefacts in mesocosm-scale 13CO2/12CO2 labelling systems: from biomass components to purified carbohydrates and dark respiration

Abstract Background Quantitative understanding of plant carbon (C) metabolism by 13CO2/12CO2-labelling studies requires absence (or knowledge) of C-isotopic contamination artefacts during tracer application and sample processing. Surprisingly, this concern has not been addressed systematically and c...

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Bibliographic Details
Main Authors: Jianjun Zhu, Regina T. Hirl, Juan C. Baca Cabrera, Rudi Schäufele, Hans Schnyder
Format: Article
Language:English
Published: BMC 2025-08-01
Series:Plant Methods
Subjects:
Atmospheric CO2 concentration
Bulk carbon
13C isotopic labelling
13C discrimination
Isotopic fractionation
C tracer
Online Access:https://doi.org/10.1186/s13007-025-01431-3
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https://doi.org/10.1186/s13007-025-01431-3

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