The Effect of Antivascular Endothelial Growth Factor on the Development of Adhesion Formation in Laparotomized Rats: Experimental Study
Aims. This study determined the effects of a single dose of bevacizumab, an antiangiogenic recombinant monoclonal antibody that specifically targets vascular endothelial growth factor (VEGF), on adhesion formation in the rat cecal abrasion model. Methodology. Thirty female Wistar albino rats (200–22...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2011/578691 |
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| author | Murat Basbug Nurullah Bulbuller Cemalettin Camci Refik Ayten Erhan Aygen Ibrahim Hanifi Ozercan Zulfu Arikanoglu Sami Akbulut |
| author_facet | Murat Basbug Nurullah Bulbuller Cemalettin Camci Refik Ayten Erhan Aygen Ibrahim Hanifi Ozercan Zulfu Arikanoglu Sami Akbulut |
| author_sort | Murat Basbug |
| collection | DOAJ |
| description | Aims. This study determined the effects of a single dose of bevacizumab, an antiangiogenic recombinant monoclonal antibody that specifically targets vascular endothelial growth factor (VEGF), on adhesion formation in the rat cecal abrasion model.
Methodology. Thirty female Wistar albino rats (200–224 g) were divided into three groups. All rats underwent laparotomy at which time cecal wall abrasion and abdominal wall injuries were induced. Group I (control) underwent only the abrasion procedure; Groups II and III received saline or bevacizumab intraperitoneally, respectively, following the abrasion. The rats were killed on postoperative day 7, and the severity of adhesions was evaluated, together with histopathological fibrosis parameters and immunohistochemical staining to identify the VEGF receptor. Results. The mean adhesion severity score in Groups I–III was 2.5±0.52, 2.4±0.69, and 0.7±0.82, respectively; the score in Group III was significantly lower than that in Groups I (P<0.001) and II (P<0.001). In the histopathological evaluation, the mean fibrosis score in Group III was significantly lower that the scores in Groups I (P<0.001) and II (P<0.001). VEGF staining of the adhesion areas in Group III was significantly lower than that in Groups I (P<0.001) and II (P<0.001). Conclusion. Bevacizumab decreases adhesion formation following laparotomy in rats by blocking VEGF receptor occupancy. |
| format | Article |
| id | doaj-art-470c944465d34817af236cb8e406180e |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-470c944465d34817af236cb8e406180e2025-02-03T01:31:24ZengWileyGastroenterology Research and Practice1687-61211687-630X2011-01-01201110.1155/2011/578691578691The Effect of Antivascular Endothelial Growth Factor on the Development of Adhesion Formation in Laparotomized Rats: Experimental StudyMurat Basbug0Nurullah Bulbuller1Cemalettin Camci2Refik Ayten3Erhan Aygen4Ibrahim Hanifi Ozercan5Zulfu Arikanoglu6Sami Akbulut7Department of General Surgery, Diyarbakir Education and Research Hospital, 21400 Diyarbakir, TurkeyDepartment of General Surgery, Faculty of Medicine, Firat University, 23100 Elazig, TurkeyDepartment of General Surgery, Faculty of Medicine, Firat University, 23100 Elazig, TurkeyDepartment of General Surgery, Faculty of Medicine, Firat University, 23100 Elazig, TurkeyDepartment of General Surgery, Faculty of Medicine, Firat University, 23100 Elazig, TurkeyDepartment of Medical Pathology, Faculty of Medicine, Firat University, 23100 Elazig, TurkeyDepartment of General Surgery, Diyarbakir Education and Research Hospital, 21400 Diyarbakir, TurkeyDepartment of General Surgery, Diyarbakir Education and Research Hospital, 21400 Diyarbakir, TurkeyAims. This study determined the effects of a single dose of bevacizumab, an antiangiogenic recombinant monoclonal antibody that specifically targets vascular endothelial growth factor (VEGF), on adhesion formation in the rat cecal abrasion model. Methodology. Thirty female Wistar albino rats (200–224 g) were divided into three groups. All rats underwent laparotomy at which time cecal wall abrasion and abdominal wall injuries were induced. Group I (control) underwent only the abrasion procedure; Groups II and III received saline or bevacizumab intraperitoneally, respectively, following the abrasion. The rats were killed on postoperative day 7, and the severity of adhesions was evaluated, together with histopathological fibrosis parameters and immunohistochemical staining to identify the VEGF receptor. Results. The mean adhesion severity score in Groups I–III was 2.5±0.52, 2.4±0.69, and 0.7±0.82, respectively; the score in Group III was significantly lower than that in Groups I (P<0.001) and II (P<0.001). In the histopathological evaluation, the mean fibrosis score in Group III was significantly lower that the scores in Groups I (P<0.001) and II (P<0.001). VEGF staining of the adhesion areas in Group III was significantly lower than that in Groups I (P<0.001) and II (P<0.001). Conclusion. Bevacizumab decreases adhesion formation following laparotomy in rats by blocking VEGF receptor occupancy.http://dx.doi.org/10.1155/2011/578691 |
| spellingShingle | Murat Basbug Nurullah Bulbuller Cemalettin Camci Refik Ayten Erhan Aygen Ibrahim Hanifi Ozercan Zulfu Arikanoglu Sami Akbulut The Effect of Antivascular Endothelial Growth Factor on the Development of Adhesion Formation in Laparotomized Rats: Experimental Study Gastroenterology Research and Practice |
| title | The Effect of Antivascular Endothelial Growth Factor on the Development of Adhesion Formation in Laparotomized Rats: Experimental Study |
| title_full | The Effect of Antivascular Endothelial Growth Factor on the Development of Adhesion Formation in Laparotomized Rats: Experimental Study |
| title_fullStr | The Effect of Antivascular Endothelial Growth Factor on the Development of Adhesion Formation in Laparotomized Rats: Experimental Study |
| title_full_unstemmed | The Effect of Antivascular Endothelial Growth Factor on the Development of Adhesion Formation in Laparotomized Rats: Experimental Study |
| title_short | The Effect of Antivascular Endothelial Growth Factor on the Development of Adhesion Formation in Laparotomized Rats: Experimental Study |
| title_sort | effect of antivascular endothelial growth factor on the development of adhesion formation in laparotomized rats experimental study |
| url | http://dx.doi.org/10.1155/2011/578691 |
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