Negative Purification Method for the Selection of Specific Antibodies from Polyclonal Antisera
We developed a protocol to remove nonspecific antibodies from polyclonal antisera by adsorption on non-target antigens immobilized on nitrocellulose membranes. This “negative” purification method is simple and provides better immunoreagents than the blocking of nonspecific antibodies in solution or...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2002-11-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/02335st06 |
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