Development and Characterization of a Ten-Plex Assay to Measure <i>Klebsiella pneumoniae</i> Antigen-Specific IgG in Human Sera

Development and Characterization of a Ten-Plex Assay to Measure <i>Klebsiella pneumoniae</i> Antigen-Specific IgG in Human Sera

<i>Klebsiella pneumoniae</i> is a leading cause of nosocomial infections, neonatal sepsis, and childhood mortality worldwide. A drastic rise in antibiotic-resistant isolates poses an urgent threat to humanity, and the World Health Organization (WHO) has classified this as a critical-prio...

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Main Authors: Luca Rovetini, Gianina Florentina Belciug, Luisa Massai, Francesca Nonne, Renzo Alfini, Heena Ranchod, Denasha L. Reddy, Mariagrazia Molfetta, Davide Oldrini, Makrina Totsika, Miren Iturriza, Ziyaad Dangor, Carlo Giannelli, Shabir A. Madhi, Francesca Micoli, Martina Carducci, Omar Rossi
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Methods and Protocols
Subjects:
<i>Klebsiella pneumoniae</i>
vaccine
immunogenicity
IgG quantification
multiplex assay
Luminex
Online Access:https://www.mdpi.com/2409-9279/8/3/52
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