Protocol for microindentation analysis of human cartilage and methacrylated gelatin hydrogels with varying stiffness in ATDC5 cell cultures

Protocol for microindentation analysis of human cartilage and methacrylated gelatin hydrogels with varying stiffness in ATDC5 cell cultures

Summary: Here, we present a protocol for evaluating mechanical properties of human cartilage specimens and methacrylated gelatin (GelMA) hydrogels with varying Young’s moduli for cultures of ATDC5 chondrocytes using microindentation. We describe steps for preparing human cartilage specimens and hydr...

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Bibliographic Details
Main Authors: Han Wang, Tianyou Kan, Lin Sun, Hanjun Li, Liao Wang, Mengning Yan, Zhifeng Yu
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Health Sciences
Clinical Protocol
Molecular Biology
Tissue Engineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724006762
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http://www.sciencedirect.com/science/article/pii/S2666166724006762

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