Surrogate Indexes of Insulin Resistance Are Affected by Sex and Gender and by the Combination of Smoking and Oral Contraceptives

Background: Surrogate indexes of insulin resistance (IR) are less expensive than the euglycemic glucose clamp. The simultaneous impact of sex and gender, smoking, and combined oral contraceptives (COC) on IR surrogate indexes was studied in a cohort of healthy young men and women (stratified in COC-...

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Main Authors: Giancarlo Tonolo, Andrea Montella, Mariangela V. Puci, Giovanni Sotgiu, Narcisa Muresu, Sara Cherchi, Mario Palermo, Giuseppe Seghieri, Flavia Franconi, Ilaria Campesi
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Diabetology
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Online Access:https://www.mdpi.com/2673-4540/5/7/50
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author Giancarlo Tonolo
Andrea Montella
Mariangela V. Puci
Giovanni Sotgiu
Narcisa Muresu
Sara Cherchi
Mario Palermo
Giuseppe Seghieri
Flavia Franconi
Ilaria Campesi
author_facet Giancarlo Tonolo
Andrea Montella
Mariangela V. Puci
Giovanni Sotgiu
Narcisa Muresu
Sara Cherchi
Mario Palermo
Giuseppe Seghieri
Flavia Franconi
Ilaria Campesi
author_sort Giancarlo Tonolo
collection DOAJ
description Background: Surrogate indexes of insulin resistance (IR) are less expensive than the euglycemic glucose clamp. The simultaneous impact of sex and gender, smoking, and combined oral contraceptives (COC) on IR surrogate indexes was studied in a cohort of healthy young men and women (stratified in COC-free women and COC users). Methods: Glycemia, insulin, C-peptide, TG, and HDL were measured in serum samples and used to calculate IGR, HOMA-IR-IR, QUICKI, FIRI, METS-IR, TG, and MCAi. Results: Men had higher BMI, glycemia, TG, METS-IR, TyG, and lower HDL than COC-free women and they had lower IGR and higher METS-IR and MCAi than COC users. TG, HDL, and TyG were lower and MCAi is higher in COC-free women than in COC users. In non-smokers, men had higher BMI and METS-IR and lower HDL than both cohorts of women. COC-free women showed a lower TyG index than men and COC women and lower TG, HDL, and IGR. MCAi was higher in COC-free women than in COC users. Smoking reduced sex and gender differences: HDL was lower in men than COC users and IGR was lower in men than COC-free women. Intra-sex differences were reported only in COC-free women: smokers had higher insulin, C-peptide, TG, and IGR and lower MCAi than non-smokers. Cluster analysis evidenced a significant separation between the sexes and smokers and non-smokers. Conclusions: Smoking leads to changes in the phenotype of both men and women, as well as COC in women; they should be considered independent variables in clinical studies given, representing a fundamental cornerstone in the personalization of prevention and care.
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spelling doaj-art-444b94bc2f88491f8a667c76dfc331592025-08-20T02:55:56ZengMDPI AGDiabetology2673-45402024-12-015767768910.3390/diabetology5070050Surrogate Indexes of Insulin Resistance Are Affected by Sex and Gender and by the Combination of Smoking and Oral ContraceptivesGiancarlo Tonolo0Andrea Montella1Mariangela V. Puci2Giovanni Sotgiu3Narcisa Muresu4Sara Cherchi5Mario Palermo6Giuseppe Seghieri7Flavia Franconi8Ilaria Campesi9Struttura Complessa di Diabetologia della Asl Gallura, 07026 Olbia, ItalyDepartment of Biomedical Sciences, University of Sassari, 07100 Sassari, ItalyClinical Epidemiology and Medical Statistics Unit, Department of Medicine, Surgery and Pharmacy, University of Sassari, 07100 Sassari, ItalyClinical Epidemiology and Medical Statistics Unit, Department of Medicine, Surgery and Pharmacy, University of Sassari, 07100 Sassari, ItalyDepartment of Humanities and Social Science, University of Sassari, 07100 Sassari, ItalyStruttura Complessa di Diabetologia della Asl Gallura, 07026 Olbia, ItalyEndocrinology and Nutrition and Replacement Diseases, Azienda Ospedaliero Universitaria di Sassari, 07100 Sassari, ItalyEpidemiology Unit, Regional Health Agency of Tuscany, 50141 Firenze, ItalyLaboratory of Sex-Gender Medicine, National Institute of Biostructures and Biosystems, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, 07100 Sassari, ItalyBackground: Surrogate indexes of insulin resistance (IR) are less expensive than the euglycemic glucose clamp. The simultaneous impact of sex and gender, smoking, and combined oral contraceptives (COC) on IR surrogate indexes was studied in a cohort of healthy young men and women (stratified in COC-free women and COC users). Methods: Glycemia, insulin, C-peptide, TG, and HDL were measured in serum samples and used to calculate IGR, HOMA-IR-IR, QUICKI, FIRI, METS-IR, TG, and MCAi. Results: Men had higher BMI, glycemia, TG, METS-IR, TyG, and lower HDL than COC-free women and they had lower IGR and higher METS-IR and MCAi than COC users. TG, HDL, and TyG were lower and MCAi is higher in COC-free women than in COC users. In non-smokers, men had higher BMI and METS-IR and lower HDL than both cohorts of women. COC-free women showed a lower TyG index than men and COC women and lower TG, HDL, and IGR. MCAi was higher in COC-free women than in COC users. Smoking reduced sex and gender differences: HDL was lower in men than COC users and IGR was lower in men than COC-free women. Intra-sex differences were reported only in COC-free women: smokers had higher insulin, C-peptide, TG, and IGR and lower MCAi than non-smokers. Cluster analysis evidenced a significant separation between the sexes and smokers and non-smokers. Conclusions: Smoking leads to changes in the phenotype of both men and women, as well as COC in women; they should be considered independent variables in clinical studies given, representing a fundamental cornerstone in the personalization of prevention and care.https://www.mdpi.com/2673-4540/5/7/50tobacco smokingcombined oral contraceptivessex and gender differencesinsulin resistanceinsulin resistance indexeshumans
spellingShingle Giancarlo Tonolo
Andrea Montella
Mariangela V. Puci
Giovanni Sotgiu
Narcisa Muresu
Sara Cherchi
Mario Palermo
Giuseppe Seghieri
Flavia Franconi
Ilaria Campesi
Surrogate Indexes of Insulin Resistance Are Affected by Sex and Gender and by the Combination of Smoking and Oral Contraceptives
Diabetology
tobacco smoking
combined oral contraceptives
sex and gender differences
insulin resistance
insulin resistance indexes
humans
title Surrogate Indexes of Insulin Resistance Are Affected by Sex and Gender and by the Combination of Smoking and Oral Contraceptives
title_full Surrogate Indexes of Insulin Resistance Are Affected by Sex and Gender and by the Combination of Smoking and Oral Contraceptives
title_fullStr Surrogate Indexes of Insulin Resistance Are Affected by Sex and Gender and by the Combination of Smoking and Oral Contraceptives
title_full_unstemmed Surrogate Indexes of Insulin Resistance Are Affected by Sex and Gender and by the Combination of Smoking and Oral Contraceptives
title_short Surrogate Indexes of Insulin Resistance Are Affected by Sex and Gender and by the Combination of Smoking and Oral Contraceptives
title_sort surrogate indexes of insulin resistance are affected by sex and gender and by the combination of smoking and oral contraceptives
topic tobacco smoking
combined oral contraceptives
sex and gender differences
insulin resistance
insulin resistance indexes
humans
url https://www.mdpi.com/2673-4540/5/7/50
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