Effect of NaCl Stress on Chlorophyll Biosynthesis, Proline, Lipid Peroxidation and Antioxidative Enzymes in Leaves of Salt-Tolerant and Salt-Sensitive Barley Cultivars

In this research, the salt tolerance of salt-tolerant (Avcı-2002) and salt-sensitive (Tokak 157/37) barley cultivars were analyzed. The seedlings were grown in a hydroponic culture containing 0, 100, 200 and 300 mM NaCl. The decrease in dry weight was more significant in Tokak 157/37 than in Avcı-20...

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Main Authors: Hakan Terzi, Mustafa Yıldız
Format: Article
Language:English
Published: Ankara University 2013-07-01
Series:Journal of Agricultural Sciences
Subjects:
Online Access:https://dergipark.org.tr/tr/download/article-file/1546506
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author Hakan Terzi
Mustafa Yıldız
author_facet Hakan Terzi
Mustafa Yıldız
author_sort Hakan Terzi
collection DOAJ
description In this research, the salt tolerance of salt-tolerant (Avcı-2002) and salt-sensitive (Tokak 157/37) barley cultivars were analyzed. The seedlings were grown in a hydroponic culture containing 0, 100, 200 and 300 mM NaCl. The decrease in dry weight was more significant in Tokak 157/37 than in Avcı-2002. On the other hand, no significant correlations were found between the altered δ-aminolevulinic acid dehydratase (ALAD) activity and the chlorophyll content. The chlorophyll and carotenoid contents in leaves of Avcı-2002 decreased significantly at only 300 mM NaCl, whereas the pigment contents of Tokak 157/37 decreased at all NaCl concentrations. The increase in proline content was significantly higher in Avcı-2002 than in Tokak 157/37 at 300 mM NaCl. The lipid peroxidation level measured in terms of malondialdehyde (MDA) content was higher in Tokak 157/37 than in Avcı-2002. Salt stress caused significant increases in superoxide dismutase (SOD), guaiacol peroxidase (POD), ascorbate peroxidase (APX) and catalase (CAT) activity. However, Avcı-2002 had relatively lower SOD and CAT activities and higher POD and APX activities than Tokak 157/37. As a result, dry weight, pigments content, proline content, lipid peroxidation and activities of POD and APX results are in good correlation with supporting Avcı-2002 being relatively salt-tolerant.
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institution Kabale University
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publishDate 2013-07-01
publisher Ankara University
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spelling doaj-art-444a7b31311a4ad6b937d49a331d20142025-08-20T03:36:03ZengAnkara UniversityJournal of Agricultural Sciences1300-75802148-92972013-07-01192798810.1501/Tarimbil_000000123245Effect of NaCl Stress on Chlorophyll Biosynthesis, Proline, Lipid Peroxidation and Antioxidative Enzymes in Leaves of Salt-Tolerant and Salt-Sensitive Barley CultivarsHakan TerziMustafa YıldızIn this research, the salt tolerance of salt-tolerant (Avcı-2002) and salt-sensitive (Tokak 157/37) barley cultivars were analyzed. The seedlings were grown in a hydroponic culture containing 0, 100, 200 and 300 mM NaCl. The decrease in dry weight was more significant in Tokak 157/37 than in Avcı-2002. On the other hand, no significant correlations were found between the altered δ-aminolevulinic acid dehydratase (ALAD) activity and the chlorophyll content. The chlorophyll and carotenoid contents in leaves of Avcı-2002 decreased significantly at only 300 mM NaCl, whereas the pigment contents of Tokak 157/37 decreased at all NaCl concentrations. The increase in proline content was significantly higher in Avcı-2002 than in Tokak 157/37 at 300 mM NaCl. The lipid peroxidation level measured in terms of malondialdehyde (MDA) content was higher in Tokak 157/37 than in Avcı-2002. Salt stress caused significant increases in superoxide dismutase (SOD), guaiacol peroxidase (POD), ascorbate peroxidase (APX) and catalase (CAT) activity. However, Avcı-2002 had relatively lower SOD and CAT activities and higher POD and APX activities than Tokak 157/37. As a result, dry weight, pigments content, proline content, lipid peroxidation and activities of POD and APX results are in good correlation with supporting Avcı-2002 being relatively salt-tolerant.https://dergipark.org.tr/tr/download/article-file/1546506barleychlorophyll biosynthesisprolinelipid peroxidationantioxidant enzymessalt tolerancearpaklorofil biyosenteziprolinlipit peroksidasyonuantioksidant enzimlertuza tolerans
spellingShingle Hakan Terzi
Mustafa Yıldız
Effect of NaCl Stress on Chlorophyll Biosynthesis, Proline, Lipid Peroxidation and Antioxidative Enzymes in Leaves of Salt-Tolerant and Salt-Sensitive Barley Cultivars
Journal of Agricultural Sciences
barley
chlorophyll biosynthesis
proline
lipid peroxidation
antioxidant enzymes
salt tolerance
arpa
klorofil biyosentezi
prolin
lipit peroksidasyonu
antioksidant enzimler
tuza tolerans
title Effect of NaCl Stress on Chlorophyll Biosynthesis, Proline, Lipid Peroxidation and Antioxidative Enzymes in Leaves of Salt-Tolerant and Salt-Sensitive Barley Cultivars
title_full Effect of NaCl Stress on Chlorophyll Biosynthesis, Proline, Lipid Peroxidation and Antioxidative Enzymes in Leaves of Salt-Tolerant and Salt-Sensitive Barley Cultivars
title_fullStr Effect of NaCl Stress on Chlorophyll Biosynthesis, Proline, Lipid Peroxidation and Antioxidative Enzymes in Leaves of Salt-Tolerant and Salt-Sensitive Barley Cultivars
title_full_unstemmed Effect of NaCl Stress on Chlorophyll Biosynthesis, Proline, Lipid Peroxidation and Antioxidative Enzymes in Leaves of Salt-Tolerant and Salt-Sensitive Barley Cultivars
title_short Effect of NaCl Stress on Chlorophyll Biosynthesis, Proline, Lipid Peroxidation and Antioxidative Enzymes in Leaves of Salt-Tolerant and Salt-Sensitive Barley Cultivars
title_sort effect of nacl stress on chlorophyll biosynthesis proline lipid peroxidation and antioxidative enzymes in leaves of salt tolerant and salt sensitive barley cultivars
topic barley
chlorophyll biosynthesis
proline
lipid peroxidation
antioxidant enzymes
salt tolerance
arpa
klorofil biyosentezi
prolin
lipit peroksidasyonu
antioksidant enzimler
tuza tolerans
url https://dergipark.org.tr/tr/download/article-file/1546506
work_keys_str_mv AT hakanterzi effectofnaclstressonchlorophyllbiosynthesisprolinelipidperoxidationandantioxidativeenzymesinleavesofsalttolerantandsaltsensitivebarleycultivars
AT mustafayıldız effectofnaclstressonchlorophyllbiosynthesisprolinelipidperoxidationandantioxidativeenzymesinleavesofsalttolerantandsaltsensitivebarleycultivars