Mechanisms of the TGF-β1/Smad3-signaling pathway in gender differences in alcoholic liver fibrosis
Abstract The TGF-β1/Smad3-signaling pathway and gender differences were investigated in alcoholic liver fibrosis. Mice were divided into female normal, female model, male normal, and male model groups. Liver injury and fibrosis were assessed using histopathology and serology. Western blotting was pe...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Journal of Physiological Sciences |
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| Online Access: | https://doi.org/10.1186/s12576-024-00901-y |
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| author | Xiaomin Hong Sanqiang Li Renli Luo Mengli Yang Junfei Wu Shuning Chen Siyu Zhu |
| author_facet | Xiaomin Hong Sanqiang Li Renli Luo Mengli Yang Junfei Wu Shuning Chen Siyu Zhu |
| author_sort | Xiaomin Hong |
| collection | DOAJ |
| description | Abstract The TGF-β1/Smad3-signaling pathway and gender differences were investigated in alcoholic liver fibrosis. Mice were divided into female normal, female model, male normal, and male model groups. Liver injury and fibrosis were assessed using histopathology and serology. Western blotting was performed to analyze the expression of relevant factors. HSC-T6 cells were divided into estradiol + saline, estradiol + ethanol, testosterone + saline, and testosterone + ethanol groups, and similar assessments were conducted in vitro. Compared with the female model group, the male model group exhibited significantly increased GPT, GOT, TNF-α, IL-6, and testosterone levels, fibrosis rate, and TGF-β1, Smad3, and PCNA expression, and significantly decreased estradiol levels and Caspase-3 expression. The apoptosis rate was higher in the estradiol + ethanol group than in the testosterone + ethanol group, although the testosterone + ethanol group exhibited significantly increased TNF-α, IL-6, Collagen-I, α-SMA, TGF-β1, Smad3, and PCNA expression, and significantly decreased Caspase-3 expression. Alcoholic liver fibrosis showed significant gender differences associated with the TGF-β1/Smad3-signaling pathway. |
| format | Article |
| id | doaj-art-43e9b00a03e642b2bceee81cedbfb35a |
| institution | Kabale University |
| issn | 1880-6562 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Physiological Sciences |
| spelling | doaj-art-43e9b00a03e642b2bceee81cedbfb35a2025-08-20T03:36:06ZengElsevierJournal of Physiological Sciences1880-65622024-02-0174111010.1186/s12576-024-00901-yMechanisms of the TGF-β1/Smad3-signaling pathway in gender differences in alcoholic liver fibrosisXiaomin Hong0Sanqiang Li1Renli Luo2Mengli Yang3Junfei Wu4Shuning Chen5Siyu Zhu6The Molecular Medicine Key Laboratory of Liver Injury and Repair, College of Basic Medicine and Forensic Medicine, Henan University of Science and TechnologyThe Molecular Medicine Key Laboratory of Liver Injury and Repair, College of Basic Medicine and Forensic Medicine, Henan University of Science and TechnologyThe Molecular Medicine Key Laboratory of Liver Injury and Repair, College of Basic Medicine and Forensic Medicine, Henan University of Science and TechnologyThe Molecular Medicine Key Laboratory of Liver Injury and Repair, College of Basic Medicine and Forensic Medicine, Henan University of Science and TechnologyThe Molecular Medicine Key Laboratory of Liver Injury and Repair, College of Basic Medicine and Forensic Medicine, Henan University of Science and TechnologyPharmaceutical Science, Monash UniversityThe Molecular Medicine Key Laboratory of Liver Injury and Repair, College of Basic Medicine and Forensic Medicine, Henan University of Science and TechnologyAbstract The TGF-β1/Smad3-signaling pathway and gender differences were investigated in alcoholic liver fibrosis. Mice were divided into female normal, female model, male normal, and male model groups. Liver injury and fibrosis were assessed using histopathology and serology. Western blotting was performed to analyze the expression of relevant factors. HSC-T6 cells were divided into estradiol + saline, estradiol + ethanol, testosterone + saline, and testosterone + ethanol groups, and similar assessments were conducted in vitro. Compared with the female model group, the male model group exhibited significantly increased GPT, GOT, TNF-α, IL-6, and testosterone levels, fibrosis rate, and TGF-β1, Smad3, and PCNA expression, and significantly decreased estradiol levels and Caspase-3 expression. The apoptosis rate was higher in the estradiol + ethanol group than in the testosterone + ethanol group, although the testosterone + ethanol group exhibited significantly increased TNF-α, IL-6, Collagen-I, α-SMA, TGF-β1, Smad3, and PCNA expression, and significantly decreased Caspase-3 expression. Alcoholic liver fibrosis showed significant gender differences associated with the TGF-β1/Smad3-signaling pathway.https://doi.org/10.1186/s12576-024-00901-yAlcoholic liver fibrosisGender differencesEstradiolTestosteroneTGF-β1/Smad3-signaling pathway |
| spellingShingle | Xiaomin Hong Sanqiang Li Renli Luo Mengli Yang Junfei Wu Shuning Chen Siyu Zhu Mechanisms of the TGF-β1/Smad3-signaling pathway in gender differences in alcoholic liver fibrosis Journal of Physiological Sciences Alcoholic liver fibrosis Gender differences Estradiol Testosterone TGF-β1/Smad3-signaling pathway |
| title | Mechanisms of the TGF-β1/Smad3-signaling pathway in gender differences in alcoholic liver fibrosis |
| title_full | Mechanisms of the TGF-β1/Smad3-signaling pathway in gender differences in alcoholic liver fibrosis |
| title_fullStr | Mechanisms of the TGF-β1/Smad3-signaling pathway in gender differences in alcoholic liver fibrosis |
| title_full_unstemmed | Mechanisms of the TGF-β1/Smad3-signaling pathway in gender differences in alcoholic liver fibrosis |
| title_short | Mechanisms of the TGF-β1/Smad3-signaling pathway in gender differences in alcoholic liver fibrosis |
| title_sort | mechanisms of the tgf β1 smad3 signaling pathway in gender differences in alcoholic liver fibrosis |
| topic | Alcoholic liver fibrosis Gender differences Estradiol Testosterone TGF-β1/Smad3-signaling pathway |
| url | https://doi.org/10.1186/s12576-024-00901-y |
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