Association between IFN-γ +874A/T and IFN-γR1 (-611A/G, +189T/G, and +95C/T) Gene Polymorphisms and Chronic Periodontitis in a Sample of Iranian Population
Background. Interferon gamma (IFN-γ) is an immune regulatory cytokine that acts through its receptor and plays important role in progression of inflammatory disease such as chronic periodontitis (CP). The purpose of this study was to determine the differences in the distribution of IFN-γ (+874A/T) a...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2015/375359 |
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| author | Zahra Heidari Hamidreza Mahmoudzadeh-Sagheb Mohammad Hashemi Somayeh Ansarimoghaddam Bita Moudi Nadia Sheibak |
| author_facet | Zahra Heidari Hamidreza Mahmoudzadeh-Sagheb Mohammad Hashemi Somayeh Ansarimoghaddam Bita Moudi Nadia Sheibak |
| author_sort | Zahra Heidari |
| collection | DOAJ |
| description | Background. Interferon gamma (IFN-γ) is an immune regulatory cytokine that acts through its receptor and plays important role in progression of inflammatory disease such as chronic periodontitis (CP). The purpose of this study was to determine the differences in the distribution of IFN-γ (+874A/T) and IFN-γR1 (-611A/G, +189T/G, and +95C/T) gene polymorphisms among CP and healthy individuals and to investigate relationships between these polymorphisms and susceptibility to CP. Materials and Methods. 310 individuals were enrolled in the study including 210 CP patients and 100 healthy controls. Single nucleotide polymorphisms at IFN-γ (+874A/T) and IFN-γR1 (-611A/G, +189T/G, and +95C/T) were analyzed by ARMS-PCR and PCR-RFLP methods. Results. The significant difference was found in genotype and allele frequency of IFN-γ (+874A/T) gene polymorphism in chronic periodontitis patients and healthy controls. The distribution of genotypes and allele frequencies for IFN-γR1 (-611A/G, +189T/G, and +95C/T) were similar among the groups and no differences in the frequencies of alleles or genotypes of IFN-γR1 genetic polymorphisms variants between case and control groups were detected. Conclusion. The finding of this study showed that IFN-γ +874A/T gene polymorphism may affect susceptibility to CP, whereas IFN-γR1 genetic polymorphisms at -611A/G, +189T/G, and +95C/T were not associated with this disease. |
| format | Article |
| id | doaj-art-43de9ce0308d426ca272e633693496f8 |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-43de9ce0308d426ca272e633693496f82025-02-03T01:00:52ZengWileyInternational Journal of Dentistry1687-87281687-87362015-01-01201510.1155/2015/375359375359Association between IFN-γ +874A/T and IFN-γR1 (-611A/G, +189T/G, and +95C/T) Gene Polymorphisms and Chronic Periodontitis in a Sample of Iranian PopulationZahra Heidari0Hamidreza Mahmoudzadeh-Sagheb1Mohammad Hashemi2Somayeh Ansarimoghaddam3Bita Moudi4Nadia Sheibak5Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan 98167-43175, IranInfectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan 98167-43175, IranCellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 98167-43175, IranDepartment of Periodontology, School of Dentistry, Zahedan University of Medical Sciences, Zahedan 98167-43175, IranInfectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan 98167-43175, IranDepartment of Histology, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167-43175, IranBackground. Interferon gamma (IFN-γ) is an immune regulatory cytokine that acts through its receptor and plays important role in progression of inflammatory disease such as chronic periodontitis (CP). The purpose of this study was to determine the differences in the distribution of IFN-γ (+874A/T) and IFN-γR1 (-611A/G, +189T/G, and +95C/T) gene polymorphisms among CP and healthy individuals and to investigate relationships between these polymorphisms and susceptibility to CP. Materials and Methods. 310 individuals were enrolled in the study including 210 CP patients and 100 healthy controls. Single nucleotide polymorphisms at IFN-γ (+874A/T) and IFN-γR1 (-611A/G, +189T/G, and +95C/T) were analyzed by ARMS-PCR and PCR-RFLP methods. Results. The significant difference was found in genotype and allele frequency of IFN-γ (+874A/T) gene polymorphism in chronic periodontitis patients and healthy controls. The distribution of genotypes and allele frequencies for IFN-γR1 (-611A/G, +189T/G, and +95C/T) were similar among the groups and no differences in the frequencies of alleles or genotypes of IFN-γR1 genetic polymorphisms variants between case and control groups were detected. Conclusion. The finding of this study showed that IFN-γ +874A/T gene polymorphism may affect susceptibility to CP, whereas IFN-γR1 genetic polymorphisms at -611A/G, +189T/G, and +95C/T were not associated with this disease.http://dx.doi.org/10.1155/2015/375359 |
| spellingShingle | Zahra Heidari Hamidreza Mahmoudzadeh-Sagheb Mohammad Hashemi Somayeh Ansarimoghaddam Bita Moudi Nadia Sheibak Association between IFN-γ +874A/T and IFN-γR1 (-611A/G, +189T/G, and +95C/T) Gene Polymorphisms and Chronic Periodontitis in a Sample of Iranian Population International Journal of Dentistry |
| title | Association between IFN-γ +874A/T and IFN-γR1 (-611A/G, +189T/G, and +95C/T) Gene Polymorphisms and Chronic Periodontitis in a Sample of Iranian Population |
| title_full | Association between IFN-γ +874A/T and IFN-γR1 (-611A/G, +189T/G, and +95C/T) Gene Polymorphisms and Chronic Periodontitis in a Sample of Iranian Population |
| title_fullStr | Association between IFN-γ +874A/T and IFN-γR1 (-611A/G, +189T/G, and +95C/T) Gene Polymorphisms and Chronic Periodontitis in a Sample of Iranian Population |
| title_full_unstemmed | Association between IFN-γ +874A/T and IFN-γR1 (-611A/G, +189T/G, and +95C/T) Gene Polymorphisms and Chronic Periodontitis in a Sample of Iranian Population |
| title_short | Association between IFN-γ +874A/T and IFN-γR1 (-611A/G, +189T/G, and +95C/T) Gene Polymorphisms and Chronic Periodontitis in a Sample of Iranian Population |
| title_sort | association between ifn γ 874a t and ifn γr1 611a g 189t g and 95c t gene polymorphisms and chronic periodontitis in a sample of iranian population |
| url | http://dx.doi.org/10.1155/2015/375359 |
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