Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model
The systemic administration of autologous bone marrow (BM) derived mononuclear cells (MNCs) is under investigation as a novel therapeutic modality for the treatment of ischemic stroke. Autologous applications raise the possibility that MNCs could potentially be stored as a banked source. There have...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/5876836 |
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| author | Bing Yang Kaushik Parsha Krystal Schaar Nikunj Satani Xiaopei Xi Jaroslaw Aronowski Sean I. Savitz |
| author_facet | Bing Yang Kaushik Parsha Krystal Schaar Nikunj Satani Xiaopei Xi Jaroslaw Aronowski Sean I. Savitz |
| author_sort | Bing Yang |
| collection | DOAJ |
| description | The systemic administration of autologous bone marrow (BM) derived mononuclear cells (MNCs) is under investigation as a novel therapeutic modality for the treatment of ischemic stroke. Autologous applications raise the possibility that MNCs could potentially be stored as a banked source. There have been no studies that investigate the effects of cryopreservation of BM-MNCs on their functional abilities in stroke models. In the present study, C57BL/6 mice were subjected to middle cerebral artery occlusion (MCAo) for 60 minutes and then divided into two treatment groups: fresh MNCs versus cryopreserved MNCs. BM-MNCs were collected at 22 hours after MCAo and were stored in liquid nitrogen for 12 months in cryopreserved MNCs group. BM-MNCs cellular viability, composition, and phenotype of the various subpopulations of mice BM-MNCs were evaluated by flow cytometry, and the behavioral recovery of stroke animals was tested with freshly harvested MNCs versus cryopreserved MNCs by corner test and ladder rung test. We found that long-term cryopreservation negatively impacts the cellular viability of bone marrow MNCs. Cryopreservation also alters the cellular composition of various subpopulations within the MNCs. However, despite the changes observed in cryopreserved cells, both fresh and frozen MNCs have similar beneficial effect on behavioral and histological outcomes. |
| format | Article |
| id | doaj-art-42346cb0fe284bffaf63f66c1c711335 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-42346cb0fe284bffaf63f66c1c7113352025-02-03T01:02:11ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/58768365876836Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke ModelBing Yang0Kaushik Parsha1Krystal Schaar2Nikunj Satani3Xiaopei Xi4Jaroslaw Aronowski5Sean I. Savitz6Stroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USAStroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USAStroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USAStroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USAStroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USAStroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USAStroke Program, Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, TX 77030, USAThe systemic administration of autologous bone marrow (BM) derived mononuclear cells (MNCs) is under investigation as a novel therapeutic modality for the treatment of ischemic stroke. Autologous applications raise the possibility that MNCs could potentially be stored as a banked source. There have been no studies that investigate the effects of cryopreservation of BM-MNCs on their functional abilities in stroke models. In the present study, C57BL/6 mice were subjected to middle cerebral artery occlusion (MCAo) for 60 minutes and then divided into two treatment groups: fresh MNCs versus cryopreserved MNCs. BM-MNCs were collected at 22 hours after MCAo and were stored in liquid nitrogen for 12 months in cryopreserved MNCs group. BM-MNCs cellular viability, composition, and phenotype of the various subpopulations of mice BM-MNCs were evaluated by flow cytometry, and the behavioral recovery of stroke animals was tested with freshly harvested MNCs versus cryopreserved MNCs by corner test and ladder rung test. We found that long-term cryopreservation negatively impacts the cellular viability of bone marrow MNCs. Cryopreservation also alters the cellular composition of various subpopulations within the MNCs. However, despite the changes observed in cryopreserved cells, both fresh and frozen MNCs have similar beneficial effect on behavioral and histological outcomes.http://dx.doi.org/10.1155/2016/5876836 |
| spellingShingle | Bing Yang Kaushik Parsha Krystal Schaar Nikunj Satani Xiaopei Xi Jaroslaw Aronowski Sean I. Savitz Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model Stem Cells International |
| title | Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model |
| title_full | Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model |
| title_fullStr | Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model |
| title_full_unstemmed | Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model |
| title_short | Cryopreservation of Bone Marrow Mononuclear Cells Alters Their Viability and Subpopulation Composition but Not Their Treatment Effects in a Rodent Stroke Model |
| title_sort | cryopreservation of bone marrow mononuclear cells alters their viability and subpopulation composition but not their treatment effects in a rodent stroke model |
| url | http://dx.doi.org/10.1155/2016/5876836 |
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