Studying concatenation of the Cas9-cleaved transgenes using barcodes

Studying concatenation of the Cas9-cleaved transgenes using barcodes

In pronuclear microinjection, the Cas9 endonuclease is employed to introduce in vivo DNA double-strand breaks at the genomic target locus or within the donor vector, thereby enhancing transgene integration. The manner by which Cas9 interacts with DNA repair factors during transgene end processing an...

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Bibliographic Details
Main Authors: A. V. Smirnov, A. N. Korablev, I. A. Serova, A. M. Yunusova, A. A. Muravyova, E. S. Valeev, N. R. Battulin
Format: Article
Language:English
Published: Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders 2025-03-01
Series:Вавиловский журнал генетики и селекции
Subjects:
crispr/cas9
pronuclear microinjection
dna barcoding
transgenic animals
dsb repair
concatemer
homologous recombination (hr)
non-homologous end-joining
nhej
mouse embryos
Online Access:https://vavilov.elpub.ru/jour/article/view/4473
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