Thio-NHS esters are non-innocent protein acylating reagents

Abstract N-Hydroxysuccinimide (NHS)-ester derivatives are widely used reagents in biological chemistry and chemical biology. Their efficacy relies critically on the exclusive chemoselectivity of activated acyl over that of the imidic acyl moieties in the succinimide. Here, through systematic structu...

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Bibliographic Details
Main Authors: Weibing Liu, Aziz Khan, Yana Demyanenko, Shabaz Mohammed, Benjamin G. Davis
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-60527-5
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