Emergence of Carbapenem-Resistant <i>bla</i><sub>POM-1</sub> Harboring <i>Pseudomonas otitidis</i> Isolated from River Water in Ghana

Emergence of Carbapenem-Resistant <i>bla</i><sub>POM-1</sub> Harboring <i>Pseudomonas otitidis</i> Isolated from River Water in Ghana

<b>Introduction:</b> <i>Pseudomonas otitidis</i>, known for carrying the <i>bla</i><sub>POM-1</sub> gene and linked to various diseases, is widely distributed. However, its prevalence in Ghana is unknown, mainly due to misidentification or inadequate r...

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Main Authors:	Frederick Ofosu Appiah, Samiratu Mahazu, Isaac Prah, Taira Kawamura, Yusuke Ota, Yohei Nishikawa, Mitsunori Yoshida, Masato Suzuki, Yoshihiko Hoshino, Toshihiko Suzuki, Tomoko Ishino, Anthony Ablordey, Ryoichi Saito
Format:	Article
Language:	English
Published:	MDPI AG 2025-01-01
Series:	Antibiotics
Subjects:	<i>Pseudomonas otitidis</i> <i>Pseudomonas aeruginosa</i> metallo-β-lactamases POM-1 carbapenems virulence factors maximum-likelihood phylogeny
Online Access:	https://www.mdpi.com/2079-6382/14/1/50
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Summary:	<b>Introduction:</b> <i>Pseudomonas otitidis</i>, known for carrying the <i>bla</i><sub>POM-1</sub> gene and linked to various diseases, is widely distributed. However, its prevalence in Ghana is unknown, mainly due to misidentification or inadequate research. In this study, for the first time, we characterized <i>P. otitidis</i> from Densu river water in Ghana. <b>Methods:</b> The antimicrobial susceptibility and whole genome characteristics of two strains (Tg_9B and BC12) were determined. The resistance and virulence features were determined using ResFinder and the VFDB database, respectively. Maximum-likelihood phylogeny was conducted based on amino acid sequences of <i>bla</i><sub>POM-1</sub> and <i>P. otitidis</i> core genomes. <b>Results:</b> The strains carried <i>bla</i><sub>POM-1</sub> on the chromosome, with only Tg_9B showing intermediate resistance to meropenem. Tg_9B had a unique genetic make-up downstream of <i>bla</i><sub>POM-1</sub>, compared with BC12 and other reference strains. Both strains harbored virulence factors able to induce pathogenicity through immune evasion. The efflux pump genes (<i>adeF</i>, <i>rsmA</i>, and <i>qacG</i>) were present in the genomes of all the strains used in this study. The amino acid sequences of <i>POM-1</i> in the strains shared a sequence homology with seven other sequences from different countries. <b>Conclusions:</b> This study highlights the emergence of <i>bla</i><sub>POM-1</sub> harboring <i>P. otitidis</i> in Ghana and affirms the conservation of <i>bla</i><sub>POM-1</sub> and <i>adeF</i>, <i>rsmA</i>, and <i>qacG</i> in the species.
ISSN:	2079-6382