Profiling of Flavonoid Constituents in Sophora davidii Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry and Evaluation of Antioxidant Activity
The dried root of Sophora davidii Kom. ex Pavol (Leguminosae, family) is a folk medicine and has long been used for the treatment of various diseases, including cough, hematochezia, sore throat, and dysentery. In this study, UHPLC-Q-Exactive Orbitrap mass spectrometry investigations were applied to...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/joch/6719888 |
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| author | Shihan Qin Xue Jiang Shani Li Qing Li Jin Huang Qingjiao He Wei Cai |
| author_facet | Shihan Qin Xue Jiang Shani Li Qing Li Jin Huang Qingjiao He Wei Cai |
| author_sort | Shihan Qin |
| collection | DOAJ |
| description | The dried root of Sophora davidii Kom. ex Pavol (Leguminosae, family) is a folk medicine and has long been used for the treatment of various diseases, including cough, hematochezia, sore throat, and dysentery. In this study, UHPLC-Q-Exactive Orbitrap mass spectrometry investigations were applied to obtain the profiling of the flavonoids in S. davidii. Finally, 108 compounds were identified, and 85 of them were firstly reported. Then, the in vitro antioxidant activity and tyrosinase activity of S. davidii were investigated using the DPPH radical scavenging ability, hydroxyl radical scavenging, and copper-reducing ability method and by measuring the rate of tyrosinase-catalyzed L-dopa oxidation. To our knowledge, this is the first study of S. davidii’s antioxidant activity, anti-tyrosinase activity, and systematic characterization of flavonoids. The current study will be of support for the usage of S. davidii in the future development of the pharmaceutical industry. |
| format | Article |
| id | doaj-art-3ed3e4528cfa44aab3787dec41270da9 |
| institution | Kabale University |
| issn | 2090-9071 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-3ed3e4528cfa44aab3787dec41270da92025-01-08T00:00:01ZengWileyJournal of Chemistry2090-90712024-01-01202410.1155/joch/6719888Profiling of Flavonoid Constituents in Sophora davidii Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry and Evaluation of Antioxidant ActivityShihan Qin0Xue Jiang1Shani Li2Qing Li3Jin Huang4Qingjiao He5Wei Cai6School of Pharmaceutical SciencesSchool of Pharmaceutical SciencesSchool of Pharmaceutical SciencesSchool of Pharmaceutical SciencesSchool of Pharmaceutical SciencesSchool of Pharmaceutical SciencesSchool of Pharmaceutical SciencesThe dried root of Sophora davidii Kom. ex Pavol (Leguminosae, family) is a folk medicine and has long been used for the treatment of various diseases, including cough, hematochezia, sore throat, and dysentery. In this study, UHPLC-Q-Exactive Orbitrap mass spectrometry investigations were applied to obtain the profiling of the flavonoids in S. davidii. Finally, 108 compounds were identified, and 85 of them were firstly reported. Then, the in vitro antioxidant activity and tyrosinase activity of S. davidii were investigated using the DPPH radical scavenging ability, hydroxyl radical scavenging, and copper-reducing ability method and by measuring the rate of tyrosinase-catalyzed L-dopa oxidation. To our knowledge, this is the first study of S. davidii’s antioxidant activity, anti-tyrosinase activity, and systematic characterization of flavonoids. The current study will be of support for the usage of S. davidii in the future development of the pharmaceutical industry.http://dx.doi.org/10.1155/joch/6719888 |
| spellingShingle | Shihan Qin Xue Jiang Shani Li Qing Li Jin Huang Qingjiao He Wei Cai Profiling of Flavonoid Constituents in Sophora davidii Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry and Evaluation of Antioxidant Activity Journal of Chemistry |
| title | Profiling of Flavonoid Constituents in Sophora davidii Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry and Evaluation of Antioxidant Activity |
| title_full | Profiling of Flavonoid Constituents in Sophora davidii Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry and Evaluation of Antioxidant Activity |
| title_fullStr | Profiling of Flavonoid Constituents in Sophora davidii Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry and Evaluation of Antioxidant Activity |
| title_full_unstemmed | Profiling of Flavonoid Constituents in Sophora davidii Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry and Evaluation of Antioxidant Activity |
| title_short | Profiling of Flavonoid Constituents in Sophora davidii Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry and Evaluation of Antioxidant Activity |
| title_sort | profiling of flavonoid constituents in sophora davidii using uhplc q exactive orbitrap mass spectrometry and evaluation of antioxidant activity |
| url | http://dx.doi.org/10.1155/joch/6719888 |
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