Insights into the tripartite interaction: effects of Arbuscular mycorrhizae and Rhizobium on root morphology, soil enzymes, and biochemical properties in pea cultivation in alluvial soils of Punjab, India

Arbuscular Mycorrhizal Fungi (AMF) and Rhizobium (RHZ) are key bio-inoculants in sustainable agriculture, known for their symbiotic relationships with plants. However, their effects on soil functions under different proportions of inorganic fertilizers are not well understood. This study, conducted...

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Main Authors: Jean Pierre Mugabo, Balkrishna S. Bhople, Arun K. Kumar, Vijay Kant Singh, Eman A. Mahmoud, Fazal Ullah, Hosam O. Elansary, Omkar Singh
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Cogent Food & Agriculture
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Online Access:https://www.tandfonline.com/doi/10.1080/23311932.2024.2366384
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author Jean Pierre Mugabo
Balkrishna S. Bhople
Arun K. Kumar
Vijay Kant Singh
Eman A. Mahmoud
Fazal Ullah
Hosam O. Elansary
Omkar Singh
author_facet Jean Pierre Mugabo
Balkrishna S. Bhople
Arun K. Kumar
Vijay Kant Singh
Eman A. Mahmoud
Fazal Ullah
Hosam O. Elansary
Omkar Singh
author_sort Jean Pierre Mugabo
collection DOAJ
description Arbuscular Mycorrhizal Fungi (AMF) and Rhizobium (RHZ) are key bio-inoculants in sustainable agriculture, known for their symbiotic relationships with plants. However, their effects on soil functions under different proportions of inorganic fertilizers are not well understood. This study, conducted during the Rabi seasons from 2019 to 2021 in alluvial soils of Punjab, India, investigates the impact of AMF and RHZ inoculation on root morphology and rhizospheric soil chemical properties in field pea (Pisum sativum L.). The findings indicate that dual inoculation with AMF and RHZ (RHZ + AMF + N50%+P50%+K100%) significantly enhances root growth and improves soil chemical properties. Despite an initial increase in pH that negatively affected micronutrient availability at 60 days after sowing (DAS), a stabilizing trend at 90 DAS was observed, leading to better availability of Fe, Cu, Mn, and Zn, along with higher Cation Exchange Capacity and macronutrient availability. This dual inoculation strategy is found to maximize profitability in terms of root morphology and soil chemical properties. Notably, lower root Cation Exchange Capacity compared to soil Cation Exchange Capacity may be due to factors like soil structure and root interactions. Principal Component Analysis (PCA) of soil parameters effectively distinguishes between treatments, showing that RHZ and AMF respond differently to various NPK proportions. For instance, treatments T3 (RHZ + N50% + P100% + K100%), T4 (RHZ + N75% + P100% + K100%), and T6 (AMF + N100% + P75% + K100%) are grouped together, while treatments T5 (AMF + N100% + P50% + K100%) and T7 (RHZ + AMF + N50% + P50% + K100%) cluster separately. This suggests that dual inoculation, especially as seen in Treatment T7, is recommended for sustained soil health and enhanced productivity.
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spelling doaj-art-3e7e18fd0bf54157adbf386dd7a4de4b2025-08-20T02:49:47ZengTaylor & Francis GroupCogent Food & Agriculture2331-19322024-12-0110110.1080/23311932.2024.2366384Insights into the tripartite interaction: effects of Arbuscular mycorrhizae and Rhizobium on root morphology, soil enzymes, and biochemical properties in pea cultivation in alluvial soils of Punjab, IndiaJean Pierre Mugabo0Balkrishna S. Bhople1Arun K. Kumar2Vijay Kant Singh3Eman A. Mahmoud4Fazal Ullah5Hosam O. Elansary6Omkar Singh7School of Agriculture, Lovely Professional University, Phagwara, IndiaRegional Research Station, Punjab Agricultural University, Ludhiana, IndiaSchool of Biosciences, Engineering and Technology, VIT Bhopal University, Sehore, IndiaDepartment of Soil Science, Punjab Agricultural University, Ludhiana, IndiaDepartment of Food Science, Faculty of Agriculture, Damietta University, Damietta, EgyptState Key Laboratory of Grassland Agro-ecosystems, College of Ecology, Lanzhou University, Lanzhou, ChinaPlant Production Department, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi ArabiaDepartment of Soil Science, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut, IndiaArbuscular Mycorrhizal Fungi (AMF) and Rhizobium (RHZ) are key bio-inoculants in sustainable agriculture, known for their symbiotic relationships with plants. However, their effects on soil functions under different proportions of inorganic fertilizers are not well understood. This study, conducted during the Rabi seasons from 2019 to 2021 in alluvial soils of Punjab, India, investigates the impact of AMF and RHZ inoculation on root morphology and rhizospheric soil chemical properties in field pea (Pisum sativum L.). The findings indicate that dual inoculation with AMF and RHZ (RHZ + AMF + N50%+P50%+K100%) significantly enhances root growth and improves soil chemical properties. Despite an initial increase in pH that negatively affected micronutrient availability at 60 days after sowing (DAS), a stabilizing trend at 90 DAS was observed, leading to better availability of Fe, Cu, Mn, and Zn, along with higher Cation Exchange Capacity and macronutrient availability. This dual inoculation strategy is found to maximize profitability in terms of root morphology and soil chemical properties. Notably, lower root Cation Exchange Capacity compared to soil Cation Exchange Capacity may be due to factors like soil structure and root interactions. Principal Component Analysis (PCA) of soil parameters effectively distinguishes between treatments, showing that RHZ and AMF respond differently to various NPK proportions. For instance, treatments T3 (RHZ + N50% + P100% + K100%), T4 (RHZ + N75% + P100% + K100%), and T6 (AMF + N100% + P75% + K100%) are grouped together, while treatments T5 (AMF + N100% + P50% + K100%) and T7 (RHZ + AMF + N50% + P50% + K100%) cluster separately. This suggests that dual inoculation, especially as seen in Treatment T7, is recommended for sustained soil health and enhanced productivity.https://www.tandfonline.com/doi/10.1080/23311932.2024.2366384Arbuscular Mycorrhizal Fungifield pearoot morphologyRhizobiumrhizospheric soilsoil chemical properties
spellingShingle Jean Pierre Mugabo
Balkrishna S. Bhople
Arun K. Kumar
Vijay Kant Singh
Eman A. Mahmoud
Fazal Ullah
Hosam O. Elansary
Omkar Singh
Insights into the tripartite interaction: effects of Arbuscular mycorrhizae and Rhizobium on root morphology, soil enzymes, and biochemical properties in pea cultivation in alluvial soils of Punjab, India
Cogent Food & Agriculture
Arbuscular Mycorrhizal Fungi
field pea
root morphology
Rhizobium
rhizospheric soil
soil chemical properties
title Insights into the tripartite interaction: effects of Arbuscular mycorrhizae and Rhizobium on root morphology, soil enzymes, and biochemical properties in pea cultivation in alluvial soils of Punjab, India
title_full Insights into the tripartite interaction: effects of Arbuscular mycorrhizae and Rhizobium on root morphology, soil enzymes, and biochemical properties in pea cultivation in alluvial soils of Punjab, India
title_fullStr Insights into the tripartite interaction: effects of Arbuscular mycorrhizae and Rhizobium on root morphology, soil enzymes, and biochemical properties in pea cultivation in alluvial soils of Punjab, India
title_full_unstemmed Insights into the tripartite interaction: effects of Arbuscular mycorrhizae and Rhizobium on root morphology, soil enzymes, and biochemical properties in pea cultivation in alluvial soils of Punjab, India
title_short Insights into the tripartite interaction: effects of Arbuscular mycorrhizae and Rhizobium on root morphology, soil enzymes, and biochemical properties in pea cultivation in alluvial soils of Punjab, India
title_sort insights into the tripartite interaction effects of arbuscular mycorrhizae and rhizobium on root morphology soil enzymes and biochemical properties in pea cultivation in alluvial soils of punjab india
topic Arbuscular Mycorrhizal Fungi
field pea
root morphology
Rhizobium
rhizospheric soil
soil chemical properties
url https://www.tandfonline.com/doi/10.1080/23311932.2024.2366384
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