Isolation, Purification, and in Vitro Hypoglycemic Activity of Pumpkin Seed Polysaccharides
After oil extraction, pumpkin seeds generate a large amount of waste meal, and polysaccharides are one of the main active components in pumpkin seed meal. To mitigate resource waste and maximize its value, pumpkin seed meal was used as raw material in this study to extract crude polysaccharides in l...
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The editorial department of Science and Technology of Food Industry
2025-05-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024050172 |
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| author | Shu CAI Cheng ZHONG Minzi LI Junyun LUO Aimei ZHOU |
| author_facet | Shu CAI Cheng ZHONG Minzi LI Junyun LUO Aimei ZHOU |
| author_sort | Shu CAI |
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| description | After oil extraction, pumpkin seeds generate a large amount of waste meal, and polysaccharides are one of the main active components in pumpkin seed meal. To mitigate resource waste and maximize its value, pumpkin seed meal was used as raw material in this study to extract crude polysaccharides in large quantities by continuous phase transition extraction device. The crude polysaccharides were then subjected to precipitation using 95% ethanol and deproteinization using the Sevage method combined with enzymatic treatment, followed by further separation and purification via DEAE-52 and Experdex-75 columns. Finally, in vitro enzyme activity inhibition assays and 3T3-L1 adipocyte experiments were conducted to investigate and compare the hypoglycemic activity and underlying mechanism of the resulted various components. The study revealed that five polysaccharide fractions (PSP, PSP-1, PSP-1-1, PSP-1-2, PSP-1-3) were obtained after purification of pumpkin seed polysaccharides. The monosaccharide composition and molar ratio of PSP-1-1, PSP-1-2 and PSP-1-3 were different. In vitro α-amylase and α-glucosidase inhibition assays showed PSP-1-1 exhibited the most potent effects with IC50 values of 0.88 mg/mL and 3.92 mg/mL, respectively. Cellular experiments demonstrated PSP-1-1 significantly enhanced the ability to uptake glucose by cells and markedly increased the high-density lipoprotein cholesterol (HDL-C) level to 257%, while reduced the levels of triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) by 48.65%, 55.91% and 50%, respectively, suggesting it could alleviate cellular insulin resistance by improving glucose and lipid metabolism. It was hypothesized that the hypoglycemic mechanism of PSP-1-1 involved promoting the expression of genes in the insulin receptor substrate (IRS-1)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB, also known as AKT) pathway, restoring normal cellular metabolism, and ultimately achieving hypoglycemic effect. This study enhances the comprehensive utilization value of pumpkin seed byproducts and provides theoretical support for the development of hypoglycemic functional foods with low toxicity and side effects. |
| format | Article |
| id | doaj-art-3e74b5010f154df498ab398db487fd66 |
| institution | OA Journals |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2025-05-01 |
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| series | Shipin gongye ke-ji |
| spelling | doaj-art-3e74b5010f154df498ab398db487fd662025-08-20T02:19:44ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-05-0146935236210.13386/j.issn1002-0306.20240501722024050172-9Isolation, Purification, and in Vitro Hypoglycemic Activity of Pumpkin Seed PolysaccharidesShu CAI0Cheng ZHONG1Minzi LI2Junyun LUO3Aimei ZHOU4Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaAfter oil extraction, pumpkin seeds generate a large amount of waste meal, and polysaccharides are one of the main active components in pumpkin seed meal. To mitigate resource waste and maximize its value, pumpkin seed meal was used as raw material in this study to extract crude polysaccharides in large quantities by continuous phase transition extraction device. The crude polysaccharides were then subjected to precipitation using 95% ethanol and deproteinization using the Sevage method combined with enzymatic treatment, followed by further separation and purification via DEAE-52 and Experdex-75 columns. Finally, in vitro enzyme activity inhibition assays and 3T3-L1 adipocyte experiments were conducted to investigate and compare the hypoglycemic activity and underlying mechanism of the resulted various components. The study revealed that five polysaccharide fractions (PSP, PSP-1, PSP-1-1, PSP-1-2, PSP-1-3) were obtained after purification of pumpkin seed polysaccharides. The monosaccharide composition and molar ratio of PSP-1-1, PSP-1-2 and PSP-1-3 were different. In vitro α-amylase and α-glucosidase inhibition assays showed PSP-1-1 exhibited the most potent effects with IC50 values of 0.88 mg/mL and 3.92 mg/mL, respectively. Cellular experiments demonstrated PSP-1-1 significantly enhanced the ability to uptake glucose by cells and markedly increased the high-density lipoprotein cholesterol (HDL-C) level to 257%, while reduced the levels of triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) by 48.65%, 55.91% and 50%, respectively, suggesting it could alleviate cellular insulin resistance by improving glucose and lipid metabolism. It was hypothesized that the hypoglycemic mechanism of PSP-1-1 involved promoting the expression of genes in the insulin receptor substrate (IRS-1)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB, also known as AKT) pathway, restoring normal cellular metabolism, and ultimately achieving hypoglycemic effect. This study enhances the comprehensive utilization value of pumpkin seed byproducts and provides theoretical support for the development of hypoglycemic functional foods with low toxicity and side effects.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024050172pumpkin seed polysaccharidesisolation and purificationenzyme activity3t3-l1 adipocyteshypoglycemic activitymechanism |
| spellingShingle | Shu CAI Cheng ZHONG Minzi LI Junyun LUO Aimei ZHOU Isolation, Purification, and in Vitro Hypoglycemic Activity of Pumpkin Seed Polysaccharides Shipin gongye ke-ji pumpkin seed polysaccharides isolation and purification enzyme activity 3t3-l1 adipocytes hypoglycemic activity mechanism |
| title | Isolation, Purification, and in Vitro Hypoglycemic Activity of Pumpkin Seed Polysaccharides |
| title_full | Isolation, Purification, and in Vitro Hypoglycemic Activity of Pumpkin Seed Polysaccharides |
| title_fullStr | Isolation, Purification, and in Vitro Hypoglycemic Activity of Pumpkin Seed Polysaccharides |
| title_full_unstemmed | Isolation, Purification, and in Vitro Hypoglycemic Activity of Pumpkin Seed Polysaccharides |
| title_short | Isolation, Purification, and in Vitro Hypoglycemic Activity of Pumpkin Seed Polysaccharides |
| title_sort | isolation purification and in vitro hypoglycemic activity of pumpkin seed polysaccharides |
| topic | pumpkin seed polysaccharides isolation and purification enzyme activity 3t3-l1 adipocytes hypoglycemic activity mechanism |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024050172 |
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