Characterization of 3,3′-iminodipropionitrile (IDPN) damaged utricle transcriptome in the adult mouse utricle
Utricle is an important vestibular sensory organ for maintaining balance. 3,3′-iminodipropionitrile (IDPN), a prototype nitrile toxin, has been reported to be neurotoxic and vestibulotoxic, and can be used to establish an in vivo damage model of vestibular dysfunction. However, the mechanism of utri...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2024.1487364/full |
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| author | Mengyao Tian Mengyao Tian Jingyuan Huang Jingyuan Huang Hairong Xiao Hairong Xiao Pei Jiang Pei Jiang Xiangyu Ma Xiangyu Ma Yanqin Lin Yanqin Lin Xujun Tang Xujun Tang Yintao Wang Yintao Wang Mingchen Dai Mingchen Dai Wei Tong Wei Tong Zixuan Ye Zixuan Ye Xia Sheng Xia Sheng Renjie Chai Renjie Chai Renjie Chai Renjie Chai Renjie Chai Renjie Chai Shasha Zhang Shasha Zhang |
| author_facet | Mengyao Tian Mengyao Tian Jingyuan Huang Jingyuan Huang Hairong Xiao Hairong Xiao Pei Jiang Pei Jiang Xiangyu Ma Xiangyu Ma Yanqin Lin Yanqin Lin Xujun Tang Xujun Tang Yintao Wang Yintao Wang Mingchen Dai Mingchen Dai Wei Tong Wei Tong Zixuan Ye Zixuan Ye Xia Sheng Xia Sheng Renjie Chai Renjie Chai Renjie Chai Renjie Chai Renjie Chai Renjie Chai Shasha Zhang Shasha Zhang |
| author_sort | Mengyao Tian |
| collection | DOAJ |
| description | Utricle is an important vestibular sensory organ for maintaining balance. 3,3′-iminodipropionitrile (IDPN), a prototype nitrile toxin, has been reported to be neurotoxic and vestibulotoxic, and can be used to establish an in vivo damage model of vestibular dysfunction. However, the mechanism of utricular HCs damage caused by IDPN is unclear. Here, we first studied mice balance behavior and HCs damage in IDPN utricle damage model, and found that IDPN injection in vivo can cause vestibular dysfunction and HCs damage, which is more pronounced than neomycin damage model. Then we used RNA-seq to characterize the transcriptome of IDPN damaged utricle in detail to identify genes and pathways that play roles in this process. We found 1,165 upregulated genes and 1,043 downregulated genes in IDPN damaged utricles, and identified that NF-κB pathway and TNF pathway may play important roles in IDPN damage model. Our study provides details of transcriptome of IDPN utricle damage model for further study of vestibular dysfunction. |
| format | Article |
| id | doaj-art-3dff67e7495346c397120ee1abb2093e |
| institution | OA Journals |
| issn | 1662-5099 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj-art-3dff67e7495346c397120ee1abb2093e2025-08-20T02:31:37ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992024-12-011710.3389/fnmol.2024.14873641487364Characterization of 3,3′-iminodipropionitrile (IDPN) damaged utricle transcriptome in the adult mouse utricleMengyao Tian0Mengyao Tian1Jingyuan Huang2Jingyuan Huang3Hairong Xiao4Hairong Xiao5Pei Jiang6Pei Jiang7Xiangyu Ma8Xiangyu Ma9Yanqin Lin10Yanqin Lin11Xujun Tang12Xujun Tang13Yintao Wang14Yintao Wang15Mingchen Dai16Mingchen Dai17Wei Tong18Wei Tong19Zixuan Ye20Zixuan Ye21Xia Sheng22Xia Sheng23Renjie Chai24Renjie Chai25Renjie Chai26Renjie Chai27Renjie Chai28Renjie Chai29Shasha Zhang30Shasha Zhang31State Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaSoutheast University, Shenzhen Research Institute, Shenzhen, ChinaState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaSoutheast University, Shenzhen Research Institute, Shenzhen, ChinaState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaSoutheast University, Shenzhen Research Institute, Shenzhen, ChinaState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaSoutheast University, Shenzhen Research Institute, Shenzhen, ChinaState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaSoutheast University, Shenzhen Research Institute, Shenzhen, ChinaState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaSoutheast University, Shenzhen Research Institute, Shenzhen, ChinaState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaSoutheast University, Shenzhen Research Institute, Shenzhen, ChinaState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaSoutheast University, Shenzhen Research Institute, Shenzhen, ChinaState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaSoutheast University, Shenzhen Research Institute, Shenzhen, ChinaState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaSoutheast University, Shenzhen Research Institute, Shenzhen, ChinaState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaSoutheast University, Shenzhen Research Institute, Shenzhen, ChinaSchool of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Environmental Health, School of Environmental Science and Engineering, Hainan University, Haikou, ChinaState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaSoutheast University, Shenzhen Research Institute, Shenzhen, ChinaCo-Innovation Center of Neuroregeneration, Nantong University, Nantong, ChinaDepartment of Otolaryngology Head and Neck Surgery, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, ChinaInstitute for Stem Cell and Regeneration, Chinese Academy of Science, Beijing, ChinaBeijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing, ChinaState Key Laboratory of Digital Medical Engineering, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Advanced Institute for Life and Health, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, ChinaSoutheast University, Shenzhen Research Institute, Shenzhen, ChinaUtricle is an important vestibular sensory organ for maintaining balance. 3,3′-iminodipropionitrile (IDPN), a prototype nitrile toxin, has been reported to be neurotoxic and vestibulotoxic, and can be used to establish an in vivo damage model of vestibular dysfunction. However, the mechanism of utricular HCs damage caused by IDPN is unclear. Here, we first studied mice balance behavior and HCs damage in IDPN utricle damage model, and found that IDPN injection in vivo can cause vestibular dysfunction and HCs damage, which is more pronounced than neomycin damage model. Then we used RNA-seq to characterize the transcriptome of IDPN damaged utricle in detail to identify genes and pathways that play roles in this process. We found 1,165 upregulated genes and 1,043 downregulated genes in IDPN damaged utricles, and identified that NF-κB pathway and TNF pathway may play important roles in IDPN damage model. Our study provides details of transcriptome of IDPN utricle damage model for further study of vestibular dysfunction.https://www.frontiersin.org/articles/10.3389/fnmol.2024.1487364/fullutricleIDPNRNA-seqneomycinhair cell damage |
| spellingShingle | Mengyao Tian Mengyao Tian Jingyuan Huang Jingyuan Huang Hairong Xiao Hairong Xiao Pei Jiang Pei Jiang Xiangyu Ma Xiangyu Ma Yanqin Lin Yanqin Lin Xujun Tang Xujun Tang Yintao Wang Yintao Wang Mingchen Dai Mingchen Dai Wei Tong Wei Tong Zixuan Ye Zixuan Ye Xia Sheng Xia Sheng Renjie Chai Renjie Chai Renjie Chai Renjie Chai Renjie Chai Renjie Chai Shasha Zhang Shasha Zhang Characterization of 3,3′-iminodipropionitrile (IDPN) damaged utricle transcriptome in the adult mouse utricle Frontiers in Molecular Neuroscience utricle IDPN RNA-seq neomycin hair cell damage |
| title | Characterization of 3,3′-iminodipropionitrile (IDPN) damaged utricle transcriptome in the adult mouse utricle |
| title_full | Characterization of 3,3′-iminodipropionitrile (IDPN) damaged utricle transcriptome in the adult mouse utricle |
| title_fullStr | Characterization of 3,3′-iminodipropionitrile (IDPN) damaged utricle transcriptome in the adult mouse utricle |
| title_full_unstemmed | Characterization of 3,3′-iminodipropionitrile (IDPN) damaged utricle transcriptome in the adult mouse utricle |
| title_short | Characterization of 3,3′-iminodipropionitrile (IDPN) damaged utricle transcriptome in the adult mouse utricle |
| title_sort | characterization of 3 3 iminodipropionitrile idpn damaged utricle transcriptome in the adult mouse utricle |
| topic | utricle IDPN RNA-seq neomycin hair cell damage |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2024.1487364/full |
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