Physiological and Antagonistic Properties of Pichia kluyveri for Curative and Preventive Treatments Against Post-Harvest Fruit Fungi
Postharvest fruit loss due to spoilage is mainly attributed to fungal infections. Synthetic chemicals can be used to preserve fruits, but they are expensive and pose risks to human health. The replacement of these chemicals by safer and cost- effective biocontrol agents is now a priority. This study...
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| Format: | Article |
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2021-07-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://journal.pan.olsztyn.pl/Physiological-and-Antagonistic-Properties-of-Pichia-kluyveri-for-Curative-and-Preventive,139278,0,2.html |
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| author | Maxwell Mewa Ngongang Heinrich Wilbur Du Plessis Chidi Silas Boredi Ucrecia Faith Hutchinson Karabo Seteno Obed Ntwampe Vincent Ifeanyi Okudoh Neil Paul Jolly |
| author_facet | Maxwell Mewa Ngongang Heinrich Wilbur Du Plessis Chidi Silas Boredi Ucrecia Faith Hutchinson Karabo Seteno Obed Ntwampe Vincent Ifeanyi Okudoh Neil Paul Jolly |
| author_sort | Maxwell Mewa Ngongang |
| collection | DOAJ |
| description | Postharvest fruit loss due to spoilage is mainly attributed to fungal infections. Synthetic chemicals can be used to preserve fruits, but they are expensive and pose risks to human health. The replacement of these chemicals by safer and cost- effective biocontrol agents is now a priority. This study investigated the physiological characteristics of Pichia kluyveri and its potential use as a biofungicide. The antagonistic effect of P. kluyveri against Botrytis cinerea and Monilinia laxa was tested on yeast peptone dextrose agar, grapes, apples, and pears. Yeast growth was variably possible at different temperatures, pH, and salinity levels. Strain-dependent antagonistic responses were observed on agar plates, where M. laxa was the more sensitive fungus to the antagonistic yeast. P. kluyveri demonstrated strong physiological properties under stressful temperature, pH, and salinity conditions. Preventive applications of P. kluyveri to apples were 95% effective against B. cinerea and 100% effective against M. laxa . Fruit type-dependent responses were evident on pears. Similarly, preventive application on grapes was also effective against the fungal pathogens studied. In general, the antagonistic responses were both fungus- and treatment- (curative and preventive) dependent. Therefore, the preventive use of P. kluyveri against post-harvest fruit-fungal infections proved to be an effective method for biological control of grapes, apples, and pears against fungal spoilage organisms Botrytis cinerea and Monilinia laxa . |
| format | Article |
| id | doaj-art-3de57db6519242998d379fc8861219d2 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2021-07-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-3de57db6519242998d379fc8861219d22025-02-03T02:39:17ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072021-07-0171324525310.31883/pjfns/139278139278Physiological and Antagonistic Properties of Pichia kluyveri for Curative and Preventive Treatments Against Post-Harvest Fruit FungiMaxwell Mewa Ngongang0Heinrich Wilbur Du Plessis1Chidi Silas Boredi2Ucrecia Faith Hutchinson3Karabo Seteno Obed Ntwampe4Vincent Ifeanyi Okudoh5Neil Paul Jolly6Post-Harvest and Agro-Processing Technologies, ARC Infruitec-Nietvoorbij (The Fruit, Vine and Wine Institute), Agricultural Research Council, South AfricaPost-Harvest and Agro-Processing Technologies, ARC Infruitec-Nietvoorbij (The Fruit, Vine and Wine Institute), Agricultural Research Council, South AfricaPost-Harvest and Agro-Processing Technologies, ARC Infruitec-Nietvoorbij (The Fruit, Vine and Wine Institute), Agricultural Research Council, South AfricaPost-Harvest and Agro-Processing Technologies, ARC Infruitec-Nietvoorbij (The Fruit, Vine and Wine Institute), Agricultural Research Council, South AfricaBioresource Engineering Research Group (BioERG), Department of Biotechnology, Cape Peninsula University of Technology, South AfricaBioresource Engineering Research Group (BioERG), Department of Biotechnology, Cape Peninsula University of Technology, South AfricaPost-Harvest and Agro-Processing Technologies, ARC Infruitec-Nietvoorbij (The Fruit, Vine and Wine Institute), Agricultural Research Council, South AfricaPostharvest fruit loss due to spoilage is mainly attributed to fungal infections. Synthetic chemicals can be used to preserve fruits, but they are expensive and pose risks to human health. The replacement of these chemicals by safer and cost- effective biocontrol agents is now a priority. This study investigated the physiological characteristics of Pichia kluyveri and its potential use as a biofungicide. The antagonistic effect of P. kluyveri against Botrytis cinerea and Monilinia laxa was tested on yeast peptone dextrose agar, grapes, apples, and pears. Yeast growth was variably possible at different temperatures, pH, and salinity levels. Strain-dependent antagonistic responses were observed on agar plates, where M. laxa was the more sensitive fungus to the antagonistic yeast. P. kluyveri demonstrated strong physiological properties under stressful temperature, pH, and salinity conditions. Preventive applications of P. kluyveri to apples were 95% effective against B. cinerea and 100% effective against M. laxa . Fruit type-dependent responses were evident on pears. Similarly, preventive application on grapes was also effective against the fungal pathogens studied. In general, the antagonistic responses were both fungus- and treatment- (curative and preventive) dependent. Therefore, the preventive use of P. kluyveri against post-harvest fruit-fungal infections proved to be an effective method for biological control of grapes, apples, and pears against fungal spoilage organisms Botrytis cinerea and Monilinia laxa .http://journal.pan.olsztyn.pl/Physiological-and-Antagonistic-Properties-of-Pichia-kluyveri-for-Curative-and-Preventive,139278,0,2.htmlbiocontrolbotrytis cinereamonilinia laxaspoilageantagonistic yeast |
| spellingShingle | Maxwell Mewa Ngongang Heinrich Wilbur Du Plessis Chidi Silas Boredi Ucrecia Faith Hutchinson Karabo Seteno Obed Ntwampe Vincent Ifeanyi Okudoh Neil Paul Jolly Physiological and Antagonistic Properties of Pichia kluyveri for Curative and Preventive Treatments Against Post-Harvest Fruit Fungi Polish Journal of Food and Nutrition Sciences biocontrol botrytis cinerea monilinia laxa spoilage antagonistic yeast |
| title | Physiological and Antagonistic Properties of Pichia kluyveri for Curative and Preventive Treatments Against Post-Harvest Fruit Fungi |
| title_full | Physiological and Antagonistic Properties of Pichia kluyveri for Curative and Preventive Treatments Against Post-Harvest Fruit Fungi |
| title_fullStr | Physiological and Antagonistic Properties of Pichia kluyveri for Curative and Preventive Treatments Against Post-Harvest Fruit Fungi |
| title_full_unstemmed | Physiological and Antagonistic Properties of Pichia kluyveri for Curative and Preventive Treatments Against Post-Harvest Fruit Fungi |
| title_short | Physiological and Antagonistic Properties of Pichia kluyveri for Curative and Preventive Treatments Against Post-Harvest Fruit Fungi |
| title_sort | physiological and antagonistic properties of pichia kluyveri for curative and preventive treatments against post harvest fruit fungi |
| topic | biocontrol botrytis cinerea monilinia laxa spoilage antagonistic yeast |
| url | http://journal.pan.olsztyn.pl/Physiological-and-Antagonistic-Properties-of-Pichia-kluyveri-for-Curative-and-Preventive,139278,0,2.html |
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