Vector for Pop-In/Pop-Out Gene Replacement in Pichia pastoris
Gene replacement in yeast is often accomplished by using a counterselectable marker such as URA3. Although ura3 strains of Pichia pastoris have been generated, these strains are inconvenient to work with because they grow slowly, even in the presence of uracil. To overcome this limitation, we have d...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2001-08-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/01312st01 |
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