Hairpin loop to hairpin loop: a full-length assembly of the ASFV genome using Oxford Nanopore long-read sequencing

Hairpin loop to hairpin loop: a full-length assembly of the ASFV genome using Oxford Nanopore long-read sequencing

Short-read assembly of the African swine fever virus (ASFV) genome is challenging due to the presence of inverted terminal repeat (ITR) and hairpin loop sequences, which often cause ambiguity in contig reconstruction. In this study, we employed Oxford Nanopore long-read sequencing to assemble a full...

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Main Authors: Poompat Phadphon, Chutima Sonthirod, Theeradej Thaweerattanasinp, Jeremy R. Shearman, Sonicha U-thoomporn, Janya Saenboonrueng, Asawin Wanitchang, Sithichoke Tangphatsornruang, Anan Jongkaewwattana, Wirulda Pootakham
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-08-01
Series:Frontiers in Microbiology
Subjects:
hairpin loop
MA-104 cells
African swine fever virus
ASFV
adaptation
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1615977/full
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https://www.frontiersin.org/articles/10.3389/fmicb.2025.1615977/full

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