Cannabinoids Regulate Bcl-2 and Cyclin D2 Expression in Pancreatic β Cells.
Recent reports have shown that cannabinoid 1 receptors (CB1Rs) are expressed in pancreatic β cells, where they induce cell death and cell cycle arrest by directly inhibiting insulin receptor activation. Here, we report that CB1Rs regulate the expression of the anti-apoptotic protein Bcl-2 and cell c...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0150981&type=printable |
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| author | Jihye Kim Kyung Jin Lee Jung Seok Kim Jun Gi Rho Jung Jae Shin Woo Keun Song Eun Kyung Lee Josephine M Egan Wook Kim |
| author_facet | Jihye Kim Kyung Jin Lee Jung Seok Kim Jun Gi Rho Jung Jae Shin Woo Keun Song Eun Kyung Lee Josephine M Egan Wook Kim |
| author_sort | Jihye Kim |
| collection | DOAJ |
| description | Recent reports have shown that cannabinoid 1 receptors (CB1Rs) are expressed in pancreatic β cells, where they induce cell death and cell cycle arrest by directly inhibiting insulin receptor activation. Here, we report that CB1Rs regulate the expression of the anti-apoptotic protein Bcl-2 and cell cycle regulator cyclin D2 in pancreatic β cells. Treatment of MIN6 and βTC6 cells with a synthetic CB1R agonist, WIN55,212-2, led to a decrease in the expression of Bcl-2 and cyclin D2, in turn inducing cell cycle arrest in G0/G1 phase and caspase-3-dependent apoptosis. Additionally, genetic deletion and pharmacological blockade of CB1Rs after injury in mice led to increased levels of Bcl-2 and cyclin D2 in pancreatic β cells. These findings provide evidence for the involvement of Bcl-2 and cyclin D2 mediated by CB1Rs in the regulation of β-cell survival and growth, and will serve as a basis for developing new therapeutic interventions to enhance β-cell function and growth in diabetes. |
| format | Article |
| id | doaj-art-3d072aa5f075401ba5fd414dc54bf3cb |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-3d072aa5f075401ba5fd414dc54bf3cb2025-08-20T02:15:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e015098110.1371/journal.pone.0150981Cannabinoids Regulate Bcl-2 and Cyclin D2 Expression in Pancreatic β Cells.Jihye KimKyung Jin LeeJung Seok KimJun Gi RhoJung Jae ShinWoo Keun SongEun Kyung LeeJosephine M EganWook KimRecent reports have shown that cannabinoid 1 receptors (CB1Rs) are expressed in pancreatic β cells, where they induce cell death and cell cycle arrest by directly inhibiting insulin receptor activation. Here, we report that CB1Rs regulate the expression of the anti-apoptotic protein Bcl-2 and cell cycle regulator cyclin D2 in pancreatic β cells. Treatment of MIN6 and βTC6 cells with a synthetic CB1R agonist, WIN55,212-2, led to a decrease in the expression of Bcl-2 and cyclin D2, in turn inducing cell cycle arrest in G0/G1 phase and caspase-3-dependent apoptosis. Additionally, genetic deletion and pharmacological blockade of CB1Rs after injury in mice led to increased levels of Bcl-2 and cyclin D2 in pancreatic β cells. These findings provide evidence for the involvement of Bcl-2 and cyclin D2 mediated by CB1Rs in the regulation of β-cell survival and growth, and will serve as a basis for developing new therapeutic interventions to enhance β-cell function and growth in diabetes.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0150981&type=printable |
| spellingShingle | Jihye Kim Kyung Jin Lee Jung Seok Kim Jun Gi Rho Jung Jae Shin Woo Keun Song Eun Kyung Lee Josephine M Egan Wook Kim Cannabinoids Regulate Bcl-2 and Cyclin D2 Expression in Pancreatic β Cells. PLoS ONE |
| title | Cannabinoids Regulate Bcl-2 and Cyclin D2 Expression in Pancreatic β Cells. |
| title_full | Cannabinoids Regulate Bcl-2 and Cyclin D2 Expression in Pancreatic β Cells. |
| title_fullStr | Cannabinoids Regulate Bcl-2 and Cyclin D2 Expression in Pancreatic β Cells. |
| title_full_unstemmed | Cannabinoids Regulate Bcl-2 and Cyclin D2 Expression in Pancreatic β Cells. |
| title_short | Cannabinoids Regulate Bcl-2 and Cyclin D2 Expression in Pancreatic β Cells. |
| title_sort | cannabinoids regulate bcl 2 and cyclin d2 expression in pancreatic β cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0150981&type=printable |
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