Isolation of PCSK9-specific nanobodies from synthetic libraries using a combined protein selection strategy

Isolation of PCSK9-specific nanobodies from synthetic libraries using a combined protein selection strategy

Abstract Nanobodies (Nbs) hold great potential to replace conventional antibodies in various biomedical applications. However, conventional methods for their discovery can be time-consuming and expensive. We have developed a reliable protein selection strategy that combines magnetic activated cell s...

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Main Authors: Apisitt Thaiprayoon, Yodpong Chantarasorn, Worrapoj Oonanant, Anongnard Kasorn, Phoomintara Longsompurana, Satita Tapaneeyakorn, Pinpunya Riangrungroj, Fabien Loison, Andrew C. Kruse, Matthew P. DeLisa, Dujduan Waraho-Zhmayev
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Scientific Reports
Subjects:
Camelid antibody
Genetic selection
High-throughput screening
Hypercholesterolemia
Synthetic antibody library
VHH single-domain antibody
Online Access:https://doi.org/10.1038/s41598-025-88032-1
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https://doi.org/10.1038/s41598-025-88032-1

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