Production and identification of monoclonal antibodies to chCD25

BALB/c mice were immunized with purified recombinant chCD25. Spleen lymphotes were fused with SP2/0 myeloma cells. Seven hybridoma cell lines (1E1, 1G1, 4H5, 5E1, 6A1, 6C2, 6C9) secreting monoclonal antibodies (McAb) were produced by three-time limited dilution method. The titers of ascetic fluids o...

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Bibliographic Details
Main Authors: WU Wei, TENG Qiao-yang, WU Jia-jun, HUANG Zhen-yu, ZHOU Ji-yong
Format: Article
Language:English
Published: Zhejiang University Press 2007-09-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/1008-9209.2007.05.0479
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