Improved injection needles facilitate germline transformation of the buckeye butterfly Junonia coenia

Germline transformation with transposon vectors is an important tool for insect genetics, but progress in developing transformation protocols for butterflies has been limited by high post-injection ova mortality. Here we present an improved glass injection needle design for injecting butterfly ova t...

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Bibliographic Details
Main Authors: Kahlia Beaudette, Tia M. Hughes, Jeffrey M. Marcus
Format: Article
Language:English
Published: Taylor & Francis Group 2014-03-01
Series:BioTechniques
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Online Access:https://www.future-science.com/doi/10.2144/000114147
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