DHPLC-Based Method for DNA Methylation Analysis of Differential Methylated Regions from Imprinted Genes

The bisulfite genomic sequencing method is one of the most widely used techniques for methylation analysis in heterogeneous unbiased PCR, amplifying for both methylated and unmethylated alleles simultaneously. However, it requires labor-intensive and time-consuming cloning and sequencing steps. In t...

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Bibliographic Details
Main Authors: P. Couvert, K. Poirier, A. Carrié, C. Chalas, P. Jouannet, C. Beldjord, T. Bienvenu, J. Chelly, A. Kerjean
Format: Article
Language:English
Published: Taylor & Francis Group 2003-02-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/03342rr06
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