NanoTag is an improved method to map interactions between DNA and proteins not requiring IgG

NanoTag is an improved method to map interactions between DNA and proteins not requiring IgG

Abstract Genome-wide profiling of DNA–protein interactions in cells can provide important information about mechanisms of gene regulation. Most current methods for genome-wide profiling of DNA-bound proteins such as ChIP-seq and CUT&Tag use conventional IgG antibodies to bind the target protein(...

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Bibliographic Details
Main Authors: Maria A. Dimitriu, Rodrigo G. Arzate-Mejía, Leonard C. Steg, Pierre-Luc Germain, Isabelle M. Mansuy
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
Subjects:
Chromatin
DNA–protein interactions
Nanobody
CUT&Tag
Epigenomics
Tn5 tagmentation
Online Access:https://doi.org/10.1038/s41598-025-13316-5
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https://doi.org/10.1038/s41598-025-13316-5

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