Breastfeeding and Neonatal Age Influence Neutrophil-Driven Ontogeny of Blood Cell Populations in the First Week of Human Life
The first few days of life are characterized by rapid external and internal changes that require substantial immune system adaptations. Despite growing evidence of the impact of this period on lifelong immune health, this period remains largely uncharted. To identify factors that may impact the traj...
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Wiley
2024-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2024/1117796 |
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| author | Sebastiano Montante Rym Ben-Othman Nelly Amenyogbe Asimenia Angelidou Anita van den Biggelaar Bing Cai Yixuan Chen Alansana Darboe Joann Diray-Arce Rebecca Ford Olubukola Idoko Amy Lee Mandy Lo Kerry McEnaney Mehrnoush Malek David Martino Geraldine Masiria Oludare A. Odumade William Pomat Casey Shannon Kinga Smolen The EPIC Consortium Al Ozonoff Peter Richmond Scott Tebbutt Ofer Levy Beate Kampmann Ryan Brinkman Tobias Kollmann |
| author_facet | Sebastiano Montante Rym Ben-Othman Nelly Amenyogbe Asimenia Angelidou Anita van den Biggelaar Bing Cai Yixuan Chen Alansana Darboe Joann Diray-Arce Rebecca Ford Olubukola Idoko Amy Lee Mandy Lo Kerry McEnaney Mehrnoush Malek David Martino Geraldine Masiria Oludare A. Odumade William Pomat Casey Shannon Kinga Smolen The EPIC Consortium Al Ozonoff Peter Richmond Scott Tebbutt Ofer Levy Beate Kampmann Ryan Brinkman Tobias Kollmann |
| author_sort | Sebastiano Montante |
| collection | DOAJ |
| description | The first few days of life are characterized by rapid external and internal changes that require substantial immune system adaptations. Despite growing evidence of the impact of this period on lifelong immune health, this period remains largely uncharted. To identify factors that may impact the trajectory of immune development, we conducted stringently standardized, high-throughput phenotyping of peripheral white blood cell (WBC) populations from 796 newborns across two distinct cohorts (The Gambia, West Africa; Papua New Guinea, Melanesia) in the framework of a Human Immunology Project Consortium (HIPC) study. Samples were collected twice from each newborn during the first week of life, first at Day of Life 0 (at birth) and then subsequently at Day of Life 1, 3, or 7 depending on the randomization group the newborn belongs to. The subsequent analysis was conducted at an unprecedented level of detail using flow cytometry and an unbiased automated gating algorithm. The results showed that WBC composition in peripheral blood changes along patterns highly conserved across populations and environments. Changes across days of life were most pronounced in the innate myeloid compartment. Breastfeeding, and at a smaller scale neonatal vaccination, were associated with changes in peripheral blood neutrophil and monocyte cell counts. Our results suggest a common trajectory of immune development in newborns and possible association with timing of breastfeeding initiation, which may contribute to immune-mediated protection from infection in early life. These data begin to outline a specific window of opportunity for interventions that could deliberately direct WBC composition, and with that, immune trajectory and thus ontogeny in early life. This trial is registered with NCT03246230. |
| format | Article |
| id | doaj-art-3ad7341bf7814ea7b8cbe60b995dd7c0 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-3ad7341bf7814ea7b8cbe60b995dd7c02025-02-03T07:23:37ZengWileyJournal of Immunology Research2314-71562024-01-01202410.1155/2024/1117796Breastfeeding and Neonatal Age Influence Neutrophil-Driven Ontogeny of Blood Cell Populations in the First Week of Human LifeSebastiano Montante0Rym Ben-Othman1Nelly Amenyogbe2Asimenia Angelidou3Anita van den Biggelaar4Bing Cai5Yixuan Chen6Alansana Darboe7Joann Diray-Arce8Rebecca Ford9Olubukola Idoko10Amy Lee11Mandy Lo12Kerry McEnaney13Mehrnoush Malek14David Martino15Geraldine Masiria16Oludare A. Odumade17William Pomat18Casey Shannon19Kinga Smolen20The EPIC Consortium21Al Ozonoff22Peter Richmond23Scott Tebbutt24Ofer Levy25Beate Kampmann26Ryan Brinkman27Tobias Kollmann28BC Cancer AgencyTelethon Kids InstituteTelethon Kids InstitutePrecision Vaccines ProgramWesfarmers Centre of Vaccines and Infectious DiseasesDepartment of PediatricsBC Cancer AgencyVaccines and Immunity ThemePrecision Vaccines ProgramPapua New Guinea Institute of Medical ResearchDepartment of Clinical ResearchDepartment of Molecular Biology and BiochemistryTelethon Kids InstitutePrecision Vaccines ProgramBC Cancer AgencyWal-yan Respiratory Research CentrePapua New Guinea Institute of Medical ResearchPrecision Vaccines ProgramPapua New Guinea Institute of Medical ResearchPROOF Centre of ExcellencePrecision Vaccines ProgramNIH, National Institute of HealthBroad Institute of MIT and HarvardDivision of PediatricsPROOF Centre of ExcellenceBroad Institute of MIT and HarvardVaccines and Immunity ThemeBC Cancer AgencyTelethon Kids InstituteThe first few days of life are characterized by rapid external and internal changes that require substantial immune system adaptations. Despite growing evidence of the impact of this period on lifelong immune health, this period remains largely uncharted. To identify factors that may impact the trajectory of immune development, we conducted stringently standardized, high-throughput phenotyping of peripheral white blood cell (WBC) populations from 796 newborns across two distinct cohorts (The Gambia, West Africa; Papua New Guinea, Melanesia) in the framework of a Human Immunology Project Consortium (HIPC) study. Samples were collected twice from each newborn during the first week of life, first at Day of Life 0 (at birth) and then subsequently at Day of Life 1, 3, or 7 depending on the randomization group the newborn belongs to. The subsequent analysis was conducted at an unprecedented level of detail using flow cytometry and an unbiased automated gating algorithm. The results showed that WBC composition in peripheral blood changes along patterns highly conserved across populations and environments. Changes across days of life were most pronounced in the innate myeloid compartment. Breastfeeding, and at a smaller scale neonatal vaccination, were associated with changes in peripheral blood neutrophil and monocyte cell counts. Our results suggest a common trajectory of immune development in newborns and possible association with timing of breastfeeding initiation, which may contribute to immune-mediated protection from infection in early life. These data begin to outline a specific window of opportunity for interventions that could deliberately direct WBC composition, and with that, immune trajectory and thus ontogeny in early life. This trial is registered with NCT03246230.http://dx.doi.org/10.1155/2024/1117796 |
| spellingShingle | Sebastiano Montante Rym Ben-Othman Nelly Amenyogbe Asimenia Angelidou Anita van den Biggelaar Bing Cai Yixuan Chen Alansana Darboe Joann Diray-Arce Rebecca Ford Olubukola Idoko Amy Lee Mandy Lo Kerry McEnaney Mehrnoush Malek David Martino Geraldine Masiria Oludare A. Odumade William Pomat Casey Shannon Kinga Smolen The EPIC Consortium Al Ozonoff Peter Richmond Scott Tebbutt Ofer Levy Beate Kampmann Ryan Brinkman Tobias Kollmann Breastfeeding and Neonatal Age Influence Neutrophil-Driven Ontogeny of Blood Cell Populations in the First Week of Human Life Journal of Immunology Research |
| title | Breastfeeding and Neonatal Age Influence Neutrophil-Driven Ontogeny of Blood Cell Populations in the First Week of Human Life |
| title_full | Breastfeeding and Neonatal Age Influence Neutrophil-Driven Ontogeny of Blood Cell Populations in the First Week of Human Life |
| title_fullStr | Breastfeeding and Neonatal Age Influence Neutrophil-Driven Ontogeny of Blood Cell Populations in the First Week of Human Life |
| title_full_unstemmed | Breastfeeding and Neonatal Age Influence Neutrophil-Driven Ontogeny of Blood Cell Populations in the First Week of Human Life |
| title_short | Breastfeeding and Neonatal Age Influence Neutrophil-Driven Ontogeny of Blood Cell Populations in the First Week of Human Life |
| title_sort | breastfeeding and neonatal age influence neutrophil driven ontogeny of blood cell populations in the first week of human life |
| url | http://dx.doi.org/10.1155/2024/1117796 |
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