Protocol to quantify the activation dynamics of tumor-associated T cells in mice by functional intravital microscopy

Summary: Tumor-associated T cells orchestrate cancer rejection after checkpoint blockade immunotherapy. T cell function depends on dynamic antigen recognition through the T cell receptor (TCR) resulting in T cell activation. Here, we present an approach to quantify the dynamics and magnitude of tumo...

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Bibliographic Details
Main Authors: Shannon N. Geels, Claire Murat, Alexander Moshensky, Shivashankar Othy, Francesco Marangoni
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724004751
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