Restriction digestion monitors facilitate plasmid construction and PCR cloning
Plasmid construction by “forced” or “directional” ligation of fragments digested with two different restriction enzymes is highly efficient, except when inhibited digestion of one site favors vector recircularization. Such failures often result because incomplete double digestion is undetected in ve...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2004-06-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/04366ST03 |
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