Effect of Inoculation with <i>Lactiplantibacillus plantarum</i> and Cellulase on the Quality of Mulberry Silage

Lactic acid bacteria (LAB) and cellulase have been used as additives to improve the fermentation quality of mulberry silage. This study investigated the dynamics of fermentation characteristics and bacterial communities during 60-day ensiling through three established treatment groups: Control (no i...

Full description

Saved in:
Bibliographic Details
Main Authors: Yingchao Sun, Yongcheng Chen, Zhiwei Huo, Guohong Liu, Xiaokai Zheng, Yayin Qi, Chunhui Ma, Fanfan Zhang
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Microorganisms
Subjects:
Online Access:https://www.mdpi.com/2076-2607/13/7/1437
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Lactic acid bacteria (LAB) and cellulase have been used as additives to improve the fermentation quality of mulberry silage. This study investigated the dynamics of fermentation characteristics and bacterial communities during 60-day ensiling through three established treatment groups: Control (no inoculation), <i>Lactiplantibacillus plantarum</i> (LP) inoculation as well as combination of <i>L. plantarum</i> and cellulase inoculation group (LPC). The results showed that compared with the Control group, the LP and LPC treatments significantly reduced the loss of dry matter, soluble carbohydrates, and crude protein (<i>p</i> < 0.05), effectively promoted the accumulation of lactic acid and acetic acid (<i>p</i> < 0.05), but significantly elevated ammonia nitrogen (NH<sub>3</sub>-N) production. Inoculation was beneficial to the stability of the bacterial community in mulberry branch and leaf silage because it can maintain a high level of beneficial bacteria (<i>Lactiplantibacillus</i>) and inhibit the growth of harmful bacteria (<i>Escherichia-Shigella</i>). The combination of the inoculation of <i>L. plantarum</i> and cellulase may improve the quality of mulberry branch silage.
ISSN:2076-2607