Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture
Organoid is a cell organization grown in a three-dimensional (3D) culture system which represents all characteristics of its origin. However, this organ-like structure requires supporting matrix to maintain its characteristics and functions. Matrigel, derived from mouse sarcoma, has often been used...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/8472712 |
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| author | Joo Hyun Jee Dong Hyeon Lee Jisu Ko Soojung Hahn Sang Yun Jeong Han Kyung Kim Enoch Park Seon Young Choi Sukin Jeong Joong Woon Lee Han-Jun Cho Min-Soo Kwon Jongman Yoo |
| author_facet | Joo Hyun Jee Dong Hyeon Lee Jisu Ko Soojung Hahn Sang Yun Jeong Han Kyung Kim Enoch Park Seon Young Choi Sukin Jeong Joong Woon Lee Han-Jun Cho Min-Soo Kwon Jongman Yoo |
| author_sort | Joo Hyun Jee |
| collection | DOAJ |
| description | Organoid is a cell organization grown in a three-dimensional (3D) culture system which represents all characteristics of its origin. However, this organ-like structure requires supporting matrix to maintain its characteristics and functions. Matrigel, derived from mouse sarcoma, has often been used as the supporting matrix for organoids, but the result may not be desirable for clinical applications because of the unidentified components from the mouse sarcoma. On the other hand, natural characteristics of collagen emphasize toxic-free friendly niche to both organoid and normal tissue. Hence, this study attempts to develop a new, collagen-based matrix that may substitute Matrigel in organoid culture. Collagen-based matrix was made, using type 1 collagen, Ham’s F12 nutrient mixture, and bicarbonate. Then, characteristics of mouse colon organoids were analyzed by morphology and quantitative messenger RNA (mRNA) expression, revealing that the mouse colon organoids grown in the collagen-based matrix and in Matrigel had quite similar morphology, specific markers, and proliferative rates. Mouse small intestine–derived organoids, stomach-derived organoids, and human colon–derived organoids were also cultured, all of which were successfully grown in the collagen-based matrix and had similar properties compared to those cultured in Matrigel. Furthermore, possibility of organoid transplantation was observed. When mouse colon organoids were transplanted with collagen matrix into the EDTA-colitis mouse model, colon organoids were successfully engrafted in damaged tissue. For that reason, the use of collagen-based matrix in organoid culture will render organoid cultivation less expensive and clinically applicable. |
| format | Article |
| id | doaj-art-394d384872e04fba917883993e2fa5b1 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-394d384872e04fba917883993e2fa5b12025-02-03T05:44:17ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/84727128472712Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid CultureJoo Hyun Jee0Dong Hyeon Lee1Jisu Ko2Soojung Hahn3Sang Yun Jeong4Han Kyung Kim5Enoch Park6Seon Young Choi7Sukin Jeong8Joong Woon Lee9Han-Jun Cho10Min-Soo Kwon11Jongman Yoo12Department of Microbiology, School of Medicine, CHA University, Seongnam-si, Gyeonggi-do 13488, Republic of KoreaCHA Organoid Research Center, School of Medicine, CHA University, Seongnam-si, Gyeonggi-do 13488, Republic of KoreaDepartment of Microbiology, School of Medicine, CHA University, Seongnam-si, Gyeonggi-do 13488, Republic of KoreaDepartment of Microbiology, School of Medicine, CHA University, Seongnam-si, Gyeonggi-do 13488, Republic of KoreaDepartment of Microbiology, School of Medicine, CHA University, Seongnam-si, Gyeonggi-do 13488, Republic of KoreaDepartment of Microbiology, School of Medicine, CHA University, Seongnam-si, Gyeonggi-do 13488, Republic of KoreaDepartment of Microbiology, School of Medicine, CHA University, Seongnam-si, Gyeonggi-do 13488, Republic of KoreaDepartment of Microbiology, School of Medicine, CHA University, Seongnam-si, Gyeonggi-do 13488, Republic of KoreaDepartment of Microbiology, School of Medicine, CHA University, Seongnam-si, Gyeonggi-do 13488, Republic of KoreaDepartment of Microbiology, School of Medicine, CHA University, Seongnam-si, Gyeonggi-do 13488, Republic of KoreaCHA Organoid Research Center, School of Medicine, CHA University, Seongnam-si, Gyeonggi-do 13488, Republic of KoreaCHA Organoid Research Center, School of Medicine, CHA University, Seongnam-si, Gyeonggi-do 13488, Republic of KoreaDepartment of Microbiology, School of Medicine, CHA University, Seongnam-si, Gyeonggi-do 13488, Republic of KoreaOrganoid is a cell organization grown in a three-dimensional (3D) culture system which represents all characteristics of its origin. However, this organ-like structure requires supporting matrix to maintain its characteristics and functions. Matrigel, derived from mouse sarcoma, has often been used as the supporting matrix for organoids, but the result may not be desirable for clinical applications because of the unidentified components from the mouse sarcoma. On the other hand, natural characteristics of collagen emphasize toxic-free friendly niche to both organoid and normal tissue. Hence, this study attempts to develop a new, collagen-based matrix that may substitute Matrigel in organoid culture. Collagen-based matrix was made, using type 1 collagen, Ham’s F12 nutrient mixture, and bicarbonate. Then, characteristics of mouse colon organoids were analyzed by morphology and quantitative messenger RNA (mRNA) expression, revealing that the mouse colon organoids grown in the collagen-based matrix and in Matrigel had quite similar morphology, specific markers, and proliferative rates. Mouse small intestine–derived organoids, stomach-derived organoids, and human colon–derived organoids were also cultured, all of which were successfully grown in the collagen-based matrix and had similar properties compared to those cultured in Matrigel. Furthermore, possibility of organoid transplantation was observed. When mouse colon organoids were transplanted with collagen matrix into the EDTA-colitis mouse model, colon organoids were successfully engrafted in damaged tissue. For that reason, the use of collagen-based matrix in organoid culture will render organoid cultivation less expensive and clinically applicable.http://dx.doi.org/10.1155/2019/8472712 |
| spellingShingle | Joo Hyun Jee Dong Hyeon Lee Jisu Ko Soojung Hahn Sang Yun Jeong Han Kyung Kim Enoch Park Seon Young Choi Sukin Jeong Joong Woon Lee Han-Jun Cho Min-Soo Kwon Jongman Yoo Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture Stem Cells International |
| title | Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture |
| title_full | Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture |
| title_fullStr | Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture |
| title_full_unstemmed | Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture |
| title_short | Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture |
| title_sort | development of collagen based 3d matrix for gastrointestinal tract derived organoid culture |
| url | http://dx.doi.org/10.1155/2019/8472712 |
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