Small sample amounts from rhizosphere of barley maintain microbial community structure and diversity revealed by total RNA sequencing

Small sample amounts from rhizosphere of barley maintain microbial community structure and diversity revealed by total RNA sequencing

Abstract Total RNA sequencing is a crucial technique in microbial ecology for profiling active microbial communities in various environments, including the rhizosphere. Since total RNA sequencing yields both 16 S and 18 S ribosomal RNA (rRNA), it is effective for taxonomic profiling of the full micr...

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Main Authors: Nikolaj L. Kindtler, Sanea Sheikh, Athanasios Zervas, Lea Ellegaard-Jensen, Louise Feld, Maria Scheel, Francisco Campuzano Jiménez, Rute R. da Fonseca, Kristian H. Laursen, Carsten S. Jacobsen, Flemming Ekelund
Format: Article
Language:English
Published: BMC 2025-06-01
Series:Plant Methods
Subjects:
Rhizosphere Microbiome
Barley (Hordeum vulgare)
Total RNA sequencing
Microbial diversity
Microbial richness
Microscaling
Online Access:https://doi.org/10.1186/s13007-025-01397-2
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https://doi.org/10.1186/s13007-025-01397-2

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