Development of a procedure for isolation, identification and quality assessment of bovine spermatids and evaluation of their fertilizing ability in vitro

Development of a procedure for isolation, identification and quality assessment of bovine spermatids and evaluation of their fertilizing ability in vitro

Intracytoplasmic spermatid injection into oocytes has limited efficiency in cattle, with no offspring generated so far, partly due to ambiguous spermatid identification. This study aimed to develop and validate a method for isolating and characterizing bovine spermatids to improve the efficiency of...

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Main Authors: Rolando Pasquariello, Francesca Di Filippo, Sai Kamal Nag Bonumallu, Fernanda Fagali Franchi, Ramona Pistucci, Federica Franciosi, Valentina Lodde, Alessandra Iannuzzi, Alberto Maria Luciano, Tiziana A. L. Brevini, Fulvio Gandolfi
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
cattle
bull testis
spermatids
intracytoplasmic spermatid injection
flow cytometry cell sorting
embryotechnology
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2025.1581019/full
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https://www.frontiersin.org/articles/10.3389/fbioe.2025.1581019/full

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