Error Analysis of Chemically Synthesized Polynucleotides
Two single-stranded polynucleotide constructs, 123 and 126 nucleotides in length, were chemically synthesized using standard phosphoramidite chemistry. Clonable, double-stranded DNA fragments about 100-bp long were prepared from the polynucleotides by primer extension with a DNA polymerase and end-t...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1998-02-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/98242st01 |
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