Protocol to process fresh human cerebral cortex biopsies for patch-clamp recording and immunostaining

Summary: Cerebral cortex biopsies enable the investigation of native developing and mature human brain tissue. Here, we present a protocol to process human cortical biopsies from the surgical theater to the laboratory. We describe steps for the preparation of viable acute slices for patch-clamp reco...

Full description

Saved in:
Bibliographic Details
Main Authors: Keimpe Wierda, Hajnalka Nyitrai, Amélie Lejeune, Ine Vlaeminck, Elke Leysen, Tom Theys, Joris de Wit, Pierre Vanderhaeghen, Baptiste Libé-Philippot
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724004787
Tags: Add Tag
No Tags, Be the first to tag this record!