Comprehensive Screening and Validation of Stable Internal Reference Genes for Accurate qRT-PCR Analysis in <i>Holotrichia parallela</i> under Diverse Biological Conditions and Environmental Stresses

Comprehensive Screening and Validation of Stable Internal Reference Genes for Accurate qRT-PCR Analysis in <i>Holotrichia parallela</i> under Diverse Biological Conditions and Environmental Stresses

<i>Holotrichia parallela</i> is among the world’s most destructive pests. For accurate qPCR and gene expression studies, the selection of stable and appropriate reference genes is crucial. However, a thorough evaluation of potential reference genes for use in <i>H. parallela</i&...

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Bibliographic Details
Main Authors: Zhongjun Gong, Jing Zhang, Qi Chen, Huiling Li, Ziqi Zhang, Yun Duan, Yueli Jiang, Tong Li, Jin Miao, Yuqing Wu
Format: Article
Language:English
Published: MDPI AG 2024-08-01
Series:Insects
Subjects:
<i>Holotrichia parallela</i>
qRT-PCR
reference gene screening
stability validation
Online Access:https://www.mdpi.com/2075-4450/15/9/661
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