Synthesis of HBC fluorophores with an electrophilic handle for covalent attachment to Pepper RNA

The fluorescent light-up aptamer (FLAP) Pepper can utilize fluorophores that are equipped with an electrophilic handle for the covalent attachment of the surrogate to the RNA. The resulting irreversibly tethered dye–RNA complexes have opened up new avenues for RNA imaging in live cells. Here, we rep...

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Bibliographic Details
Main Authors: Raphael Bereiter, Ronald Micura
Format: Article
Language:English
Published: Beilstein-Institut 2025-04-01
Series:Beilstein Journal of Organic Chemistry
Subjects:
Online Access:https://doi.org/10.3762/bjoc.21.56
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